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MELK Antibody (4F10X4)

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Western Blot: MELK Antibody (4F10X4) [NBP3-16211] - Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (NBP3-16211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
4F10X4
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MELK Antibody (4F10X4) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MELK (NP_055606.1). MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MELK
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for MELK Antibody (4F10X4)

  • EC 2.7.11
  • EC 2.7.11.1
  • hMELK
  • HPK38
  • KIAA0175pEg3 kinase
  • maternal embryonic leucine zipper kinase
  • MELK
  • PK38
  • Protein kinase PK38

Background

MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant, GST fused MELK protein by Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MELK