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MELK Antibody (2G2) - BSA Free

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Immunocytochemistry/Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunocytochemistry/ Immunofluorescence: MELK Antibody (2G2) [NBP2-37481] - Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MELK mouse mAb with DAB staining.
Flow Cytometry: MELK Antibody (2G2) [NBP2-37481] - Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).
Immunohistochemistry: MELK Antibody (2G2) [NBP2-37481] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MELK mouse mAb with DAB staining.
ELISA: MELK Antibody (2G2) [NBP2-37481] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
2G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37481-0.025ml
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MELK Antibody (2G2) - BSA Free Summary

Immunogen
Synthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MELK
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
This antibody is Cytof ready.
Theoretical MW
74.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for MELK Antibody (2G2) - BSA Free

  • EC 2.7.11
  • EC 2.7.11.1
  • hMELK
  • HPK38
  • KIAA0175pEg3 kinase
  • maternal embryonic leucine zipper kinase
  • MELK
  • PK38
  • Protein kinase PK38

Background

MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant, GST fused MELK protein by Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol MELK