![Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Western-Blot-NBP2-67400-img0010.jpg "Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SI16-04 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Mcl-1 aa 101-150 / 350. (SwissProt: Q07820 Human; SwissProt: P97287 Mouse; SwissProt: Q9Z1P3 Rat) |
| Localization | Membrane, Cytoplasm, Mitochondrion, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MCL1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MCL1 |
![Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunocytochemistry-Immunofluorescence-NBP2-67400-img0008.jpg "Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Mcl-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunohistochemistry-Paraffin-NBP2-67400-img0009.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Mcl-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of Jurkat cells with MCL1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Flow-Cytometry-NBP2-67400-img0001.jpg "Flow Cytometry: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of Jurkat cells with MCL1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of MCL1 on different lysates using anti-MCL1 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Mouse heart](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Western-Blot-NBP2-67400-img0007.jpg "Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of MCL1 on different lysates using anti-MCL1 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Mouse heart")
![Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Western-Blot-NBP2-67400-img0011.jpg "Western Blot: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Western blot analysis of Mcl-1 on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunocytochemistry-Immunofluorescence-NBP2-67400-img0002.jpg "Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunocytochemistry-Immunofluorescence-NBP2-67400-img0003.jpg "Immunocytochemistry/Immunofluorescence: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Staining MCL1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human breast tissue using anti-MCL1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunohistochemistry-Paraffin-NBP2-67400-img0004.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human breast tissue using anti-MCL1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human kidney tissue using anti-MCL1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunohistochemistry-Paraffin-NBP2-67400-img0005.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human kidney tissue using anti-MCL1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human pancreas tissue using anti-MCL1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Mcl-1-Antibody-SI16-04-Immunohistochemistry-Paraffin-NBP2-67400-img0006.jpg "Immunohistochemistry-Paraffin: Mcl-1 Antibody (SI16-04) [NBP2-67400] - Analysis of paraffin-embedded human pancreas tissue using anti-MCL1 antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")