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M-CSF Antibody [Unconjugated]

 (14 Publications)
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Recombinant Mouse M‑CSF (Catalog # 416-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M‑CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse M‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF416). The ND50 is typically 0.015-0.035 µg/mL.
Recombinant Mouse M‑CSF (Catalog # 416-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF416
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF416-SP
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M-CSF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse M-CSF
Lys33-Glu262
Accession # Q3U4F9
Specificity
Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human M‑CSF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CSF1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Neutralization 0.015-0.035 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF416 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSF Antibody [Unconjugated]

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells, and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O-glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82%, and 81% aa identity with corresponding regions of rat, dog, cow, and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for M-CSF Antibody (AF416)(14)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, In Vivo, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC
(4)
In Vivo
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF416 Applications Species
Xu, H;Lotfy, P;Gelb, S;Pragana, A;Hehnly, C;Shipley, FB;Zawadzki, ME;Cui, J;Deng, L;Taylor, M;Webb, M;Lidov, HGW;Andermann, ML;Chiu, IM;Ordovas-Montanes, J;Lehtinen, MK; A collaboration between immune cells and the choroid plexus epithelium in brain inflammation bioRxiv : the preprint server for biology 2023-08-08 [PMID: 37609192] (IHC, Mouse) IHC Mouse
A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain Progress in neurobiology, 2023-01-13;0(0):102405. 2023-01-13 [PMID: 36646299] (IHC, Mouse) IHC Mouse
J Cheng, X Ma, C Li, R Ullah, X Wang, J Long, Z Yuan, S Liu, J Fu, Z Chen, Y Shen, YD Zhou Diet-induced inflammation in the anterior paraventricular thalamus induces compulsive sucrose-seeking Nature Neuroscience, 2022-08-01;25(8):1009-1013. 2022-08-01 [PMID: 35915173] (In Vivo, Mouse) In Vivo Mouse
I De, V Maklakova, S Litscher, MM Boyd, LC Klemm, Z Wang, C Kendziorsk, LS Collier Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages Journal of Neuroinflammation, 2021-07-19;18(1):162. 2021-07-19 [PMID: 34281564] (IHC, Mouse) IHC Mouse
Alicia Bellomo, Isabelle Mondor, Lionel Spinelli, Marine Lagueyrie, Benjamin J. Stewart, Nicolas Brouilly et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages Immunity 2020-07-01 [PMID: 32562599]
Bonnardel J, T'Jonck W, Gaublomme D et al.. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche Immunity. 10/15/2019 [PMID: 31561945]

Details:
Citation used the Alexa Fluor 647 format of this antibody.
LJ Zhou, J Peng, YN Xu, WJ Zeng, J Zhang, X Wei, CL Mai, ZJ Lin, Y Liu, M Murugan, UB Eyo, AD Umpierre, WJ Xin, T Chen, M Li, H Wang, JR Richardson, Z Tan, XG Liu, LJ Wu Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain Cell Rep, 2019-06-25;27(13):3844-3859.e6. 2019-06-25 [PMID: 31242418] (ICC, IHC, Neutralization, Western Blot, Mouse) ICC, IHC, Neutralization, Western Blot Mouse
Isabelle Mondor, Myriam Baratin, Marine Lagueyrie, Lisa Saro, Sandrine Henri, Rebecca Gentek et al. Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche Immunity 2019-06-01 [PMID: 31053503]
Travis M. Rotterman, Erica T Akhter, Alicia R. Lane, Kathryn P. MacPherson, Violet V. Garcia, Malú G. Tansey et al. Spinal motor circuit synaptic plasticity after peripheral nerve injury depends on microglia activation and a CCR2 mechanism The Journal of Neuroscience 2019-03-04 [PMID: 30833511]
Gushchina S, Pryce G, Yip PK et al. Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal loss Glia 8/25/2018 [PMID: 30144320]
Show All 14 Publications.

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Bioinformatics

Gene Symbol CSF1
Uniprot