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Recombinant Mouse M-CSF Protein

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Recombinant Mouse M-CSF Protein (Catalog # 416-ML) has a molecular weight (MW) of 50.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.
Recombinant Mouse M-CSF (416-ML) stimulates cell proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner. The ED50 for this effect is 0.5-3 ng/mL.

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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse M-CSF Protein Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5‑3 ng/mL.
Source
E. coli-derived mouse M-CSF protein
Lys33-Glu262, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Csf1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
29 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
416-ML in the following application:

Publications
Read Publications using
416-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse M-CSF Protein

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF protein can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

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Publications for M-CSF (416-ML)(640)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Feline, Transgenic Mouse.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.


Filter By Application
Bioassay
(554)
Cell Culture
(77)
Differentiation
(8)
In Vivo
(2)
Tissue Culture
(1)
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Filter By Species
Human
(4)
Mouse
(623)
Rat
(3)
Feline
(2)
Transgenic Mouse
(17)
All Species
Showing Publications 1 - 10 of 640. Show All 640 Publications.
Publications using 416-ML Applications Species
Yang, X;Xu, X;Zhong, Q;Cui, H;Xu, J;Wei, W; Impact of T-2 toxin on intestinal inflammation and transcriptional regulation of inflammatory response in mouse macrophages Biochemistry and biophysics reports 2024-12-01 [PMID: 39483178] (Bioassay, Mouse) Bioassay Mouse
McBride, MA;Caja, KR;Patil, TK;Owen, AM;Luan, L;Bohannon, JK;Hernandez, A;Stothers, CL;Trenary, IA;Rahim, M;Young, JD;Calcutt, MW;Stephens, VR;Davis, X;Oliver, MA;Hao, D;Si, C;McRae, M;Nguyen, KK;Davis, NS;Wang, J;Patil, NK;Sherwood, ER; Immunoresponsive Gene 1 Facilitates TLR4-agonist-Induced Augmentation of Innate Antimicrobial Immunity Journal of leukocyte biology 2024-10-01 [PMID: 39351765] (Bioassay, Mouse, Transgenic Mouse) Bioassay Mouse, Transgenic Mouse
Scafidi, A;Lind-Holm Mogensen, F;Campus, E;Pailas, A;Neumann, K;Legrave, N;Bernardin, F;Pereira, SL;Antony, PMA;Nicot, N;Mittelbronn, M;Grünewald, A;Nazarov, PV;Poli, A;Van Dyck, E;Michelucci, A; Metformin impacts the differentiation of mouse bone marrow cells into macrophages affecting tumour immunity Heliyon 2024-09-30 [PMID: 39315158] (Bioassay, Mouse) Bioassay Mouse
Koroth, J;Karkache, IY;Vu, EK;Mansky, KC;Bradley, EW; CD11B + CD36 + cells are bone anabolic macrophages that limit age-associated bone loss bioRxiv : the preprint server for biology 2024-09-14 [PMID: 39314303] (Bioassay, Mouse) Bioassay Mouse
Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S; Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models bioRxiv : the preprint server for biology 2024-09-01 [PMID: 39257734] (Bioassay, Mouse) Bioassay Mouse
Zhenqiang Yao, Akram Ayoub, Venkatesan Srinivasan, Jun Wu, Churou Tang, Rong Duan, Aleksa Milosavljevic, Lianping Xing, Frank H. Ebetino, Alison J. Frontier, and Brendan F. Boyce Hydroxychloroquine and a low antiresorptive activity bisphosphonate conjugate prevent and reverse ovariectomy-induced bone loss in mice through dual antiresorptive and anabolic effects Bone Res 2024-09-05 [PMID: 39231935] (Bioassay, Mouse, Transgenic Mouse) Bioassay Mouse, Transgenic Mouse
Shu, Y;Tan, Z;Pan, Z;Chen, Y;Wang, J;He, J;Wang, J;Wang, Y; Inhibition of inflammatory osteoclasts accelerates callus remodeling in osteoporotic fractures by enhancing CGRP+TrkA+ signaling Cell death and differentiation 2024-09-02 [PMID: 39223264] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Dong, S;Ge, J;Meng, Q;Yuan, T;Wang, Y;Li, Y;Lu, Q;Song, W;Li, Z;Sun, S; Crebanine mitigates glucocorticoid-induced osteonecrosis of the femoral head by restoring bone remodelling homeostasis via attenuating oxidative stress Journal of cellular and molecular medicine 2024-08-01 [PMID: 39205463] (Bioassay, Mouse) Bioassay Mouse
Wang, L;Hu, H;Ko, CC; Osteoclast-Driven Polydopamine-to-Dopamine Release: An Upgrade Patch for Polydopamine-Functionalized Tissue Engineering Scaffolds Journal of functional biomaterials 2024-07-29 [PMID: 39194649] (Bioassay, Mouse) Bioassay Mouse
Eijken, M;Krautzberger, AM;Scholze-Wittler, M;Boers-Sijmons, B;Koedam, M;Kosiol, B;Schrewe, H;van Leeuwen, JP;van der Eerden, BC; Vasorin-deficient mice display disturbed vitamin D and mineral homeostasis in combination with a low bone mass phenotype Bone reports 2024-09-01 [PMID: 39157725] (Bioassay, Mouse) Bioassay Mouse
Show All 640 Publications.

Reviews for M-CSF (416-ML) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 416-ML:
Filter by Applications
CellProlif
(1)
Stem/Immune cell maintenance or differentiation
(1)
All Applications
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Images Ratings Applications Species Date Details
  5
reviewed by:
Walter Jackson
CellProlif 02/22/2021
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Summary

ApplicationCell Proliferation
LotME4819091
CommentsIt worked well in the proliferation and differentiation of mouse bone marrow cells in vitro.
Stem/Immune cell maintenance or differentiation  M-CSF 416-ML
Enlarge
5
reviewed by:
Verified Customer
Stem/Immune cell maintenance or differentiation 09/09/2019
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Summary

ApplicationStem/Immune cell maintenance or differentiation
LotME4619021
CommentsExcellent activity in cell culture. Effective in differentiating mouse bone marrow cells into BMDMs. Adhesion and cell health are very good for this timepoint. Price was also acceptable for the product, given comparables.

Comments

CommentsPreparing murine IFNAR-/- bone marrow macrophages for viral infection/entry studies.

FAQs for M-CSF (416-ML) (0)

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Walter Jackson
02/22/2021
Application: CellProlif
Species:

Verified Customer
09/09/2019
Application: Stem/Immune cell maintenance or differentiation
Species:

Bioinformatics

Gene Symbol Csf1
Uniprot