![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54) [NBP3-32492] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32492) at 1/1,000 dilution. Lane 1: MG-63 cell lysate (20 ug/Lane) Lane 2: Mouse cerebellum tissue lysate (30 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32492_rabbit-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-mab-278202415212441.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54) [NBP3-32492] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32492) at 1/1,000 dilution. Lane 1: MG-63 cell lysate (20 ug/Lane) Lane 2: Mouse cerebellum tissue lysate (30 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | JE36-54 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human Lysine (K)-specific Demethylase 4AKDM4A/JMJD2A aa 551-650 / 1,064. (Uniprot: O75164) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KDM4A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 121 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NBP3-32492)Find related products by research area.
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| Gene Symbol | KDM4A |
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