![Western Blot: LRPAP Antibody (009) [NBP2-90513] - Anti-LRPAP rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Western-Blot-NBP2-90513-img0003.jpg "Western Blot: LRPAP Antibody (009) [NBP2-90513] - Anti-LRPAP rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clone | 009 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LRPAP (Accession#: NP_038615.2; Gln 29-Leu 360). |
| Specificity | No cross-reactivity in ELISA with: Human LRPAP1 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LRPAP1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LRPAP1 |
![Immunocytochemistry/Immunofluorescence: LRPAP Antibody (009) [NBP2-90513] - Immunofluorescence staining of mouse LRPAP in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse LRPAP monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Endoplasmic reticulum.](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-90513-img0002.jpg "Immunocytochemistry/Immunofluorescence: LRPAP Antibody (009) [NBP2-90513] - Immunofluorescence staining of mouse LRPAP in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse LRPAP monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nerve cells.](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0005.jpg "Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nerve cells.")
![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nephric tubule.](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0004.jpg "Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nephric tubule.")
![Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513] - Mouse LRPAP was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate0.5 ul anti-Mouse LRPAP rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse LRPAP rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunoprecipitation-NBP2-90513-img0006.jpg "Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513] - Mouse LRPAP was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate0.5 ul anti-Mouse LRPAP rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse LRPAP rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")