Novus Biologicals products are now on bio-techne.com

LPCAT2 Antibody

Images

 
Western Blot: LPCAT2 Antibody [NBP1-88921] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: LPCAT2 Antibody [NBP1-88921] - Staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-88921 antibody. Corresponding LPCAT2 RNA-seq data are presented for the same tissues.
Western Blot: LPCAT2 Antibody [NBP1-88921] - Analysis in human thyroid gland tissue.
Immunocytochemistry/ Immunofluorescence: LPCAT2 Antibody [NBP1-88921] - Staining of murine cell line RAW264.7. Image provided by product review by verified customer.
Immunohistochemistry-Frozen: LPCAT2 Antibody [NBP1-88921] - Staining of LPCAT2 in rat neuronal tissue using anti-LPCAT2 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human skeletal muscle shows very weak positivity in myocytes.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human small intestine shows weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human testis shows weak granular cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: LPCAT2 Antibody [NBP1-88921] - Staining of human thyroid gland shows strong granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

LPCAT2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITEEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPCAT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen Validated from verified customer review
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.
Control Peptide
LPCAT2 Protein (NBP1-88921PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-88921 in the following applications:

Publications
Read Publications using
NBP1-88921 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32140075).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LPCAT2 Antibody

  • 1-alkylglycerophosphocholine O-acetyltransferase
  • Acetyl-CoA:lyso-PAF acetyltransferase
  • Acetyl-CoA:lyso-platelet-activating factor acetyltransferase
  • acyl-CoA:lysophosphatidylcholine acyltransferase 2
  • acyltransferase like 1
  • Acyltransferase-like 1
  • AGPAT11
  • AYTL1
  • AYTL1LysoPAFAT
  • DKFZp686H22112
  • EC 2.3.1
  • EC 2.3.1.-
  • EC 2.3.1.23
  • EC 2.3.1.67,1-acylglycerophosphocholine O-acyltransferase
  • FLJ20481
  • LPC acyltransferase 2
  • LPCAT1
  • LPCAT2
  • LPCAT-2
  • Lyso-PAF acetyltransferase
  • LysoPC acyltransferase 2
  • lysophosphatidylcholine acyltransferase 2
  • lyso-platelet-activating factor (PAF) acetyltransferase

Background

LPCAT2 encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The encoded protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The enzyme may localize to the endoplasmic reticulum and the Golgi. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-41952
Species: Hu, Po
Applications: ICC/IF, IHC, WB
NBP1-88923
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
MAB5018
Species: Hu
Applications: IP, WB
NBP3-04752
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
MAB2669
Species: Hu
Applications: IHC, Simple Western, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-57898
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-31344
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-796
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-92440
Species: Hu
Applications: IHC, IHC-P
NBP2-27104
Species: Hu, Mu, Pm
Applications: IHC, IHC-P, WB
NBP1-05950
Species: Hu, Mu
Applications: ICC/IF, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP2-73621
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC/IF
NBP1-82236
Species: Hu
Applications: IHC, IHC-P, KD, WB
DPLG70
Species: Hu
Applications: ELISA
NBP1-32452
Species: Hu, Mu, Rt
Applications: WB
H00009261-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, Simple Western, WB

Publications for LPCAT2 Antibody (NBP1-88921)(5)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(3)
All Applications
Filter By Species
Mouse
(2)
Rat
(1)
All Species

Reviews for LPCAT2 Antibody (NBP1-88921) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-88921:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen LPCAT2 NBP1-88921
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Rat 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat neuronal tissue
SpeciesRat
  4
reviewed by:
Martin Helley
ICC Mouse 02/12/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedRAW264.7 cells
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LPCAT2 Antibody (NBP1-88921). (Showing 1 - 1 of 1 FAQs).

  1. Do you have an estimated band size for the LPCAT2 antibody (NBP1-88921) for Western blotting?
    • According to Uniprot, human LPCAT2 has a predicted molecular weight of approximately 60kDa. Please see UniProt entry Q7L5N7.

Secondary Antibodies

 

Isotype Controls

Additional LPCAT2 Products

Research Areas for LPCAT2 Antibody (NBP1-88921)

Find related products by research area.

Blogs on LPCAT2

There are no specific blogs for LPCAT2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
02/20/2015
Application: IHC-Fr
Species: Rat

Martin Helley
02/12/2013
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol LPCAT2