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Lipocalin-2/NGAL Antibody [Unconjugated]

 (14 Publications)
 (1 Review)
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Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipocalin-2/NGAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by ...read more
Visualization of expression of selected proteins by Western blot analysis. Protein extracts were prepared from livers of WT and Lcn2-/- mice that were subjected to indicated diets for 4 or 6 weeks (n=3 animals/group). ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3508
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Catalog# & Formulation Size Price
Carrier Free
AF3508-SP
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Lipocalin-2/NGAL Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
Gln21-Asn198
Accession # P30152
Specificity
Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LCN2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Rat Lipocalin-2/NGAL Antibody (Catalog # AF3508)
ELISA Detection: Rat Lipocalin-2/NGAL Biotinylated Antibody (Catalog # BAF3508)
Standard: Recombinant Rat Lipocalin-2/NGAL (Catalog # 3508-LC)
Reviewed Applications
Read 1 Review rated 5
using
AF3508 in the following applications:
Publications
Read Publications using
AF3508 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody [Unconjugated]

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (KD = 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

  1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  2. Devireddy, L.R. et al. (2001) Science 293:829.
  3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  5. Flo, T.H. et al. (2004) Nature 432:917.
  6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
  8. Mishra, J. et al. (2005) Lancet 365:1231.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipocalin-2/NGAL Antibody (AF3508)(14)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 4 applications: ELISA Development, IHC, IHC-Fr, Western Blot.


Filter By Application
ELISA Development
(1)
IHC
(2)
IHC-Fr
(1)
Western Blot
(7)
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Mouse
(4)
Rat
(3)
Transgenic Mouse
(3)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3508 Applications Species
Kessel, JC;Weiskirchen, R;Schröder, SK; Expression Analysis of Lipocalin 2 (LCN2) in Reproductive and Non-Reproductive Tissues of Esr1-Deficient Mice International journal of molecular sciences 2023-05-25 [PMID: 37298232] (Western Blot, IHC, Transgenic Mouse) Western Blot, IHC Transgenic Mouse
J Wang, Z Zhang, J Guan, HC Tung, J Xie, H Huang, Y Chen, M Xu, S Ren, S Li, M Zhang, D Yang, W Xie Hepatocyte estrogen sulfotransferase inhibition protects female mice from Concanavalin A-induced T cell-mediated hepatitis independent of estrogens The Journal of Biological Chemistry, 2023-02-15;0(0):103026. 2023-02-15 [PMID: 36796516] (IHC, Western Blot, Mouse, Transgenic Mouse) IHC, Western Blot Mouse, Transgenic Mouse
C Poggel, T Adams, R Janzen, A Hofmann, O Hardt, E Roeb, SK Schröder, CG Tag, M Roderfeld, R Weiskirche Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology Biomedicines, 2022-09-06;10(9):. 2022-09-06 [PMID: 36140299] (Western Blot, Mouse) Western Blot Mouse
JY Lim, WW Jung, W Kim, KS Moon, D Sul Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide Scientific Reports, 2022-04-27;12(1):6837. 2022-04-27 [PMID: 35477741] (Western Blot, Rat) Western Blot Rat
P Klüber, SK Meurer, J Lambertz, R Schwarz, S Zechel-Gra, T Braunschwe, S Hurka, E Domann, R Weiskirche Depletion of Lipocalin 2 (LCN2) in Mice Leads to Dysbiosis and Persistent Colonization with Segmented Filamentous Bacteria International Journal of Molecular Sciences, 2021-12-05;22(23):. 2021-12-05 [PMID: 34884961] (Western Blot, Mouse, Transgenic Mouse) Western Blot Mouse, Transgenic Mouse
Asimakopoulou A, Vucur M, Luedde T et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma Cancers (Basel) 3/19/2019 [PMID: 30893876]
Erawan Borkham-Kamphorst, Eddy Van de Leur, Steffen K. Meurer, Eva M. Buhl, Ralf Weiskirchen N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting Frontiers in Pharmacology 2018-04-25 [PMID: 29755357]
J Lambertz, T Berger, TW Mak, J van Helden, R Weiskirche Lipocalin-2 in Fructose-Induced Fatty Liver Disease Front Physiol, 2017-11-28;8(0):964. 11/28/2017 [PMID: 29234288]
A Asimakopou, E Borkham-Ka, EV de Leur, R Weiskirche Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis Data Brief, 2017-07-01;13(0):644-649. 2017-07-01 [PMID: 28725667] (Western Blot, Mouse) Western Blot Mouse
Josef G. Heuer, Shannon M. Harlan, Derek D. Yang, Dianna L. Jaqua, Jeffrey S. Boyles, Jonathan M. Wilson et al. Role of TGF-alpha in the progression of diabetic kidney disease American Journal of Physiology-Renal Physiology 2017-06-01 [PMID: 28249836]
Show All 14 Publications.

Review for Lipocalin-2/NGAL Antibody (AF3508) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF3508:
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WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Rat 01/07/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21457438
SpeciesRat

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 Verified Customer
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Application: WB
Species: Rat
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Bioinformatics

Gene Symbol LCN2
Uniprot