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Lipase A Antibody [HRP]

 
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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-54155AF350
Alexa Fluor 405
NBP1-54155AF405
Alexa Fluor 488
NBP1-54155AF488
Alexa Fluor 532
NBP1-54155AF532
Alexa Fluor 594
NBP1-54155AF594
Alexa Fluor 647
NBP1-54155AF647
Alexa Fluor 700
NBP1-54155AF700
Alexa Fluor 750
NBP1-54155AF750
Allophycocyanin
NBP1-54155APC
Biotin
NBP1-54155B
CoraFluor 1
NBP1-54155CL1
DyLight 350
NBP1-54155UV
DyLight 405
NBP1-54155V
DyLight 488
NBP1-54155G
DyLight 550
NBP1-54155R
DyLight 594
NBP1-54155DL594
DyLight 650
NBP1-54155C
DyLight 680
NBP1-54155FR
DyLight 755
NBP1-54155IR
FITC
NBP1-54155F
Janelia Fluor 525
NBP1-54155JF525
Janelia Fluor 549
NBP1-54155JF549
Janelia Fluor 585
NBP1-54155JF585
Janelia Fluor 635
NBP1-54155JF635
Janelia Fluor 646
NBP1-54155JF646
Janelia Fluor 669
NBP1-54155JF669
PE
NBP1-54155PE
PerCP
NBP1-54155PCP
Unconjugated
NBP1-54155
mFluor Violet 450 SE
NBP1-54155MFV450
mFluor Violet 500 SE
NBP1-54155MFV500
mFluor Violet 610 SE
NBP1-54155MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-54155
BSA Free
NBP1-54155SS
BSA Free
NBP1-54155
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Lipase A Antibody [HRP] Summary

Immunogen
Partial recombinant protein made to an internal region of the human Lipase A protein (within residues 150-300). [Swiss-Prot P38571]
Localization
Lysosome.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIPA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen sequence has 82% identity to pig and 73% identity to rat and cow.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Lipase A Antibody [HRP]

  • Acid cholesteryl ester hydrolase
  • CESD
  • Cholesteryl esterase
  • EC 3.1.1
  • EC 3.1.1.13
  • LALCESDcholesterol ester hydrolase
  • LIPA
  • Lipase A
  • lipase A, lysosomal acid, cholesterol esterase
  • lysosomal acid lipase
  • lysosomal acid lipase/cholesteryl ester hydrolase
  • Sterol esterase

Background

Lipase A is a critical enzyme for metabolism, sequestration and degradation of cholesterol. When LIPA is not effective in liver and kidney cells, lipoprotein cholesterol accumulates and leads to atherosclerosis, inflammation, Type 2 diabetes, and chronic kidney disease. In Wolman disease, deficiency of LIPA causes buildup of cholesterol in spleen, liver, bone marrow, intestine, adrenal glands and lymph nodes, which eventually causes such toxicity in children affected that they rarely live past 1 year of age. Intracellularly, LIPA mediates the uptake of cholesterol by the endosomal/lysosomal compartment, and hydrolyzes cholesterol esterases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Lipase A Antibody
[HRP]
NBP1-54155H
Lipase A Antibody [HRP]
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIPA