LIM Kinase 1 Antibody (1B2) - Azide and BSA Free

Images

 
Western Blot: LIM Kinase 1 Antibody (1B2) [H00003984-M02] - LIMK1 monoclonal antibody (M02), clone 1B2 Analysis of LIMK1 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (1B2) [H00003984-M02] - Analysis of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml.
Sandwich ELISA: LIM Kinase 1 Antibody (1B2) [H00003984-M02] - Detection limit for recombinant GST tagged LIMK1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
1B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

LIM Kinase 1 Antibody (1B2) - Azide and BSA Free Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
LIMK1 (NP_002305, 548 a.a. ~ 647 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD
Specificity
LIMK1 (1B2)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LIMK1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA and IHC-P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LIM Kinase 1 Antibody (1B2) - Azide and BSA Free

  • EC 2.7.11.1
  • LIM domain kinase 1
  • LIM Kinase 1
  • LIM motif-containing protein kinase
  • LIMK
  • LIMK1
  • LIMK-1
  • LIMKLIMK-1

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LIMK1
Entrez
OMIM
Uniprot