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LARG Antibody

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human LARG Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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LARG Antibody Summary

Immunogen
E. coli-derived recombinant human LARG
Ile154-Leu575
Accession # Q9NZN5
Specificity
Detects endogenous human LARG in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARHGEF12
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using AF4737.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LARG Antibody

  • ARHGC
  • ARHGEF12
  • DKFZp686O2372
  • KIAA0382PRO2792
  • LARG
  • LARGleukemia-associated rho guanine nucleotide exchange factor
  • Leukemia-associated RhoGEF
  • Rho guanine nucleotide exchange factor (GEF) 12
  • rho guanine nucleotide exchange factor 12

Background

LARG (Leukemia-associated RhoGEF, also known as Rho guanine nucleotide exchange factor 12) is a 173 kDa member of the DH‑domain containing RhoGEF family of molecules. It is ubiquitously expressed, and known to interact with semaphorin-activated B class plexins, thereby activating Rho-dependent pathways. Human LARG is 1544 amino acids (aa) in length. It contains one PZD domain that binds to plexin (aa 72‑151), an RGSL region (aa 368‑558), and a RhoGEF domain (aa 788‑975). There are at least two potential splice forms. One shows a deletion of aa 48‑66 with an 8 aa poly-Lys substitution for the C-terminal 960 amino acids. A second shows an insertion of 9 aa between Thr1093 and Asp1094, accompanied by a 6 aa substitution for the C-terminal 131 amino acids. Over aa 154‑575, human LARG is 90% aa identical to mouse LARG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LARG Antibody (AF4737)(2)

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Bioinformatics

Gene Symbol ARHGEF12
Uniprot