Reactivity | HuSpecies Glossary |
Applications | WB |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant human LARG Ile154-Leu575 Accession # Q9NZN5 |
Specificity | Detects endogenous human LARG in Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | ARHGEF12 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
LARG (Leukemia-associated RhoGEF, also known as Rho guanine nucleotide exchange factor 12) is a 173 kDa member of the DH‑domain containing RhoGEF family of molecules. It is ubiquitously expressed, and known to interact with semaphorin-activated B class plexins, thereby activating Rho-dependent pathways. Human LARG is 1544 amino acids (aa) in length. It contains one PZD domain that binds to plexin (aa 72‑151), an RGSL region (aa 368‑558), and a RhoGEF domain (aa 788‑975). There are at least two potential splice forms. One shows a deletion of aa 48‑66 with an 8 aa poly-Lys substitution for the C-terminal 960 amino acids. A second shows an insertion of 9 aa between Thr1093 and Asp1094, accompanied by a 6 aa substitution for the C-terminal 131 amino acids. Over aa 154‑575, human LARG is 90% aa identical to mouse LARG.
Secondary Antibodies |
Isotype Controls |
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