KRAS Antibody - Azide and BSA Free

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Genetic Strategies: Western Blot: KRAS Antibody [NBP3-03659] - Analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat ...read more
Western Blot: Rabbit Polyclonal KRAS Antibody [NBP3-03659] - Analysis of various lysates, using KRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunocytochemistry/ Immunofluorescence: KRAS Antibody - Azide and BSA Free [NBP3-03659] - Immunofluorescence analysis of L929 cells using [KO Validated] KRAS Rabbit pAb (A12704) at dilution of 100 (40x lens). Secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

KRAS Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-188 of human KRAS (NP_203524.1). DEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCII
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRAS
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Knockout Validated
  • Western Blot 1:1000 - 1:5000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for KRAS Antibody - Azide and BSA Free

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KRAS Antibody (NBP3-03659) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRAS