![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0010.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 17 amino acids near the center of human Klotho. The immunogen is located within amino acids 410-460 of Klotho. Amino Acid Sequence: SGTTKRRDDAKYMYYLK |
| Specificity | Three isoforms of Klotho are known to exist. Human Klotho has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD). Mouse Klotho has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD). Rat Klotho has one isoform (1014aa, 117kD). NBP1-76511 can detect human, mouse and rat. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KL |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Theoretical MW | 116 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||
| Control Peptide |
|
||
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
![Simple Western CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Simple_Western_17480.jpg)
![Western Blot CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/CD117_AF1356_Western_Blot_18637.jpg)
![Immunohistochemistry CD117/c-kit Antibody [Unconjugated]](https://images.novusbio.com/images2/SCF_R_AF1356_Immunohistochemistry_9746.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Klotho Antibody (NBP1-76511)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 20 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-76511-img0002.jpg "Immunocytochemistry/Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 20 ug/ml.")
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-NBP1-76511-img0008.jpg "Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY license.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - ARPE-19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin-3a, hypoxia + AAV-p53 mutant infection and hypoxia + miRNA-34a inhibitor. Western blot assay of Klotho in ARPE-19 cells was performed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Klotho Antibody - BSA Free-Western Blot-NBP1-76511-img0011.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - ARPE-19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin-3a, hypoxia + AAV-p53 mutant infection and hypoxia + miRNA-34a inhibitor. Western blot assay of Klotho in ARPE-19 cells was performed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362/) licensed under a CC-BY license.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0007.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0009.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0003.jpg "Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.")
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Staining of rat heart tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0004.jpg "Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Staining of rat heart tissue with antibody at 2.5 ug/ml.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P < .001, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. H, RT‐PCR was performed to measure the expression of miRNA‐34a. **P < .01, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. I, FFA was performed to measure the leakage of CNV. J, The leakage of CNV was analysed. K, ICGA was performed to measure the area of CNV. L, The area of CNV was analysed. **P < .01, ***P < .001, compared with the CNV 7‐d group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-310202416171454.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P < .001, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. H, RT‐PCR was performed to measure the expression of miRNA‐34a. **P < .01, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. I, FFA was performed to measure the leakage of CNV. J, The leakage of CNV was analysed. K, ICGA was performed to measure the area of CNV. L, The area of CNV was analysed. **P < .01, ***P < .001, compared with the CNV 7‐d group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - microrna‐34a inhibits the expression of Klotho. ARPE‐19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin‐3a, hypoxia + AAV‐p53 mutant infection & hypoxia + miRNA‐34a inhibitor. A, Western blot assay of Klotho in ARPE‐19 cells was performed. B, The relative protein level of Klotho compared with the GAPDH level was analysed. ***P < .001, hypoxia group vs normal group. ###P < .001, ##P < .01, compared with the hypoxia group. %%P < .01, hypoxia + digoxin + nutlin‐3a group vs hypoxia + digoxin group. C, Schematic of the Klotho 3' UTR with the WT or mutated putative‐binding site of miRNA‐34a inserted into a luciferase (Luc) reporter. D, qRT‐PCR analysis of Klotho mRNA expression in HEK293T cells transfected with miRNA‐34a mimic for 24 h. **P < .01, miRNA‐34a mimic group vs control group. E, Western blot of Klotho in HEK293T cells transfected with a miRNA‐34a mimic for 24 h was performed. F, Immunoblotted Klotho was quantified & normalized to GAPDH. ***P < .001, miRNA‐34a mimic group vs control group. G Relative luciferase activity was detected in HEK293T cells co‐transfected with plasmids containing firefly luciferase & wild‐type (WT) or mutant (Mut) Klotho 3′‐UTR & miRNA‐34a mimic for 24 h. The luciferase activity values were normalized to Renilla reniformis luciferase (TK‐RL) activity. **P < .01, Luc‐Klotho 3′ UTR WT group vs vector group. Statistically non‐significant (NS), Luc‐Klotho 3′ UTR mutant group vs vector group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-310202416154634.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - microrna‐34a inhibits the expression of Klotho. ARPE‐19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin‐3a, hypoxia + AAV‐p53 mutant infection & hypoxia + miRNA‐34a inhibitor. A, Western blot assay of Klotho in ARPE‐19 cells was performed. B, The relative protein level of Klotho compared with the GAPDH level was analysed. ***P < .001, hypoxia group vs normal group. ###P < .001, ##P < .01, compared with the hypoxia group. %%P < .01, hypoxia + digoxin + nutlin‐3a group vs hypoxia + digoxin group. C, Schematic of the Klotho 3' UTR with the WT or mutated putative‐binding site of miRNA‐34a inserted into a luciferase (Luc) reporter. D, qRT‐PCR analysis of Klotho mRNA expression in HEK293T cells transfected with miRNA‐34a mimic for 24 h. **P < .01, miRNA‐34a mimic group vs control group. E, Western blot of Klotho in HEK293T cells transfected with a miRNA‐34a mimic for 24 h was performed. F, Immunoblotted Klotho was quantified & normalized to GAPDH. ***P < .001, miRNA‐34a mimic group vs control group. G Relative luciferase activity was detected in HEK293T cells co‐transfected with plasmids containing firefly luciferase & wild‐type (WT) or mutant (Mut) Klotho 3′‐UTR & miRNA‐34a mimic for 24 h. The luciferase activity values were normalized to Renilla reniformis luciferase (TK‐RL) activity. **P < .01, Luc‐Klotho 3′ UTR WT group vs vector group. Statistically non‐significant (NS), Luc‐Klotho 3′ UTR mutant group vs vector group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - High phytate-induced renal phosphate wasting is independent of FGF23.(A) The renal expression of downregulated genes associated with Ca2+ & phosphate homeostasis in rats fed control or phytate-supplemented diets (n = 8 per group). (B) Results of immunoblot analyses & protein levels of renal aKlotho, NHERF1, & NaPi-2a after 2 weeks for rats fed control, HP- LCa2+ , & HP-HCa2+ diets. (C) The levels of renal proteins were quantified using ImageJ software (n = 6 per group). (D, E) Time course analysis of serum levels of intact FGF23 (D) & C-terminal FGF23 (E) in rats fed control, HP-LCa2+ , & HP-HCa2+ diets. (F) Representative immunohistochemical staining of EDTA-decalcified femur sections for FGF23 from rats fed control, HP-LCa2+ , & HP-HCa2+ diets. All data are presented as the mean ± SD of each group (n = 3–8 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32271147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-310202416171414.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - High phytate-induced renal phosphate wasting is independent of FGF23.(A) The renal expression of downregulated genes associated with Ca2+ & phosphate homeostasis in rats fed control or phytate-supplemented diets (n = 8 per group). (B) Results of immunoblot analyses & protein levels of renal aKlotho, NHERF1, & NaPi-2a after 2 weeks for rats fed control, HP- LCa2+ , & HP-HCa2+ diets. (C) The levels of renal proteins were quantified using ImageJ software (n = 6 per group). (D, E) Time course analysis of serum levels of intact FGF23 (D) & C-terminal FGF23 (E) in rats fed control, HP-LCa2+ , & HP-HCa2+ diets. (F) Representative immunohistochemical staining of EDTA-decalcified femur sections for FGF23 from rats fed control, HP-LCa2+ , & HP-HCa2+ diets. All data are presented as the mean ± SD of each group (n = 3–8 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32271147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![SCF/c-kit Ligand [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "SCF/c-kit Ligand [Unconjugated]")
![Immunohistochemistry FGF-23 Antibody (283507) [Unconjugated]](https://images.novusbio.com/images2/FGF23_MAB26291_Immunohistochemistry_17626.jpg)
![Immunohistochemistry FGF-23 Antibody (283507) [Unconjugated]](https://images.novusbio.com/images/mab26291_mouse-fgf-23-mab-clone-283507-41202412512640.jpg)
![Western Blot FGF-23 Antibody (283507) [Unconjugated]](https://images.novusbio.com/images/mab26291_mouse-fgf-23-mab-clone-283507-41202412514876.jpg)
![SDS-PAGE IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_345.jpg)
![Bioactivity IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_347.jpg)
![SEC-MALS IL-3 [Unconjugated]](https://images.novusbio.com/images/203-il_recombinant-human-il-3-protein-sec-mals-133202482656..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity Flt-3 Ligand/FLT3L [Unconjugated]](https://images.novusbio.com/images/308-fk_recombinant-human-flt-3-ligand-flt3l-protein-bioactivity-2132023173432.jpg)
![Bioactivity Thrombopoietin/THPO [Unconjugated]](https://images.novusbio.com/images/protein/288-tpncf_recombinant-human-thrombopoietin-ns0-expressed-protein-cf-bioactivity-272020135047.jpg)
![Immunohistochemistry CD34 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD34_AF4117_Immunohistochemistry_6824.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/DATA_Erythropoietin_MEP00_ELISA_970.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]](https://images.novusbio.com/images/antibody/mab812_human-flt-3-flk-2-mab-clone-66903-flow-cytometry-3112023131157..jpg)
![Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]](https://images.novusbio.com/images/antibody/mab812_human-flt-3-flk-2-mab-clone-66903-flow-cytometry-3112023131255..jpg)
![SEC-MALS IL-7 [Unconjugated]](https://images.novusbio.com/images/207-il_recombinant-human-il-7-protein-sec-mals-123202493447..jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")