Kif4A Antibody

Images

 
Western Blot: Kif4A Antibody [NBP2-17056] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: Kif4A Antibody [NBP2-17056] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Kif4A Antibody [NBP2-17056] - TOV-112D xenograft, using KIF4A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Kif4A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Kif4A. The exact sequence is proprietary.
Localization
Nucleus matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF4A
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (82%), Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Kif4A Antibody

  • chromokinesin-A
  • chromosome-associated kinesin KIF4A
  • FLJ12530
  • FLJ12655
  • FLJ14204
  • FLJ20631
  • HSA271784
  • KIF4G1
  • KIF4KIF4-G1
  • kinesin family member 4A

Background

Kinesins, such as KIF4A, are microtubule-based motor proteins that generate directional movement along microtubules. They are involved in many crucial cellular processes, including cell division (Zhu and Jiang, 2005 [PubMed 15625105]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Kif4A Products

Array NBP2-17056

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Bioinformatics

Gene Symbol KIF4A
Uniprot