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![Western Blot: Kif4A Antibody [NBP3-35106] - Western blot analysis of various lysates using Kif4A Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241252857.jpg "Western Blot: Kif4A Antibody [NBP3-35106] - Western blot analysis of various lysates using Kif4A Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Human stomach using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-10122024120688.jpg)
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241252836.jpg)
![Immunohistochemistry: Kif4A Antibody [NBP3-35106] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-10122024120624.jpg)
![Immunocytochemistry/ Immunofluorescence: Kif4A Antibody [NBP3-35106] - Immunofluorescence analysis of HeLa cells using Kif4A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35106_rabbit-kif4a-pab-101220241157269.jpg)
![Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Western-Blot-NBP3-16748-img0004.jpg "Western Blot: Kif4A Antibody (4A9P2) [NBP3-16748] - Western blot analysis of extracts of various cell lines, using Kif4A antibody (NBP3-16748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded mouse testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0003.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded rat testis using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0001.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody (4A9P2) [NBP3-16748] - Immunohistochemistry of paraffin-embedded human colon using Kif4A Rabbit mAb (NBP3-16748) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Kif4A-Antibody-4A9P2-Immunohistochemistry-Paraffin-NBP3-16748-img0002.jpg)
![Western Blot: Kif4A Antibody [NBP1-83721] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Kif4A-Antibody-Western-Blot-NBP1-83721-img0005.jpg "Western Blot: Kif4A Antibody [NBP1-83721] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP1-83721] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83721-img0006.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody [NBP1-83721] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunohistochemistry-Paraffin-NBP1-83721-img0004.jpg)
![Western Blot: Kif4A Antibody [NBP1-83720] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Kif4A-Antibody-Western-Blot-NBP1-83720-img0006.jpg "Western Blot: Kif4A Antibody [NBP1-83720] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP1-83720] - Staining of human cell line A-431 shows localization to nucleoplasm & cytokinetic bridge.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83720-img0007.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody [NBP1-83720] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunohistochemistry-Paraffin-NBP1-83720-img0004.jpg)
![Western Blot: Kif4A Antibody [NBP2-17056] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Western-Blot-NBP2-17056-img0004.jpg "Western Blot: Kif4A Antibody [NBP2-17056] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody diluted by 1:1000.")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP2-17056] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17056-img0003.jpg)
![Immunohistochemistry-Paraffin: Kif4A Antibody [NBP2-17056] - TOV-112D xenograft, using KIF4A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunohistochemistry-Paraffin-NBP2-17056-img0002.jpg)
![Western Blot: Kif4A Antibody (JE38-91) [NBP3-32502] - Western blot analysis of Kif4A on different lysates with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: SW480 cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32502_rabbit-kif4a-mab-je38-91-27820241631627.jpg "Western Blot: Kif4A Antibody (JE38-91) [NBP3-32502] - Western blot analysis of Kif4A on different lysates with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: SW480 cell lysate (20 ug/Lane) Lane 5: Mouse thymus tissue lysate (40 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Predicted band size: 140 kDa Observed band size: 160 kDa Exposure time: 6 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Kif4A Antibody (JE38-91) [NBP3-32502] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Kif4A antibody (NBP3-32502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32502_rabbit-kif4a-mab-je38-91-29820241492699.jpg)
![Western Blot: Kif4A Antibody [NB100-57512] - Detection of Human KIF4A on HeLa whole cell lysate using NB100-57512. KIF4A was also immunoprecipitated by rabbit anti-KIF4A antibody NB100-57511.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Western-Blot-NB100-57512-img0001.jpg "Western Blot: Kif4A Antibody [NB100-57512] - Detection of Human KIF4A on HeLa whole cell lysate using NB100-57512. KIF4A was also immunoprecipitated by rabbit anti-KIF4A antibody NB100-57511.")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1053-img0001.jpg "Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.")
![Western Blot: Kif4A Antibody (SR1195) [NBP3-22228] - Western blot analysis using NBP3-22228 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22228_rabbit-kif4a-mab-sr1195-5720231649426.jpg "Western Blot: Kif4A Antibody (SR1195) [NBP3-22228] - Western blot analysis using NBP3-22228 in HeLa cell lysate.")
![Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP2-56589] - Staining of human cell line SiHa shows localization to nucleoplasm & cytokinetic bridge.](http://images.novusbio.com/fullsize/Kif4A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56589-img0001.jpg "Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NBP2-56589] - Staining of human cell line SiHa shows localization to nucleoplasm & cytokinetic bridge.")