Ki67/MKI67 Antibody - BSA Free

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NB110-89719	Applications	Species
Hill AA, Kim M, Ruiz DFZ et al. Defective intestinal repair after short-term high fat diet due to loss of efferocytosis bioRxiv 2019-10-02 (ICC/IF, Mouse)	ICC/IF	Mouse
Ju-Kyung Jeong, Tak-Heun Kim, Hwajung Choi, Eui-Sic Cho Impaired breakdown of Herwig's epithelial root sheath disturbs tooth root development. Developmental dynamics : an official publication of the American Association of Anatomists 2023-10-18 [PMID: 37850829]
Jo A, Green A, Medina JE et al. Inaugurating High-Throughput Profiling of Extracellular Vesicles for Earlier Ovarian Cancer Detection Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-07-23 [PMID: 37485618] (ICC/IF, Human)	ICC/IF	Human
Jo A, Green A, Medina JE et al. Profiling extracellular vesicles in circulation enables the early detection of ovarian cancer bioRxiv : the preprint server for biology 2023-01-20 [PMID: 36711872] (ICC/IF, Mouse)	ICC/IF	Mouse
AA Hill, M Kim, DF Zegarra-Ru, LC Chang, KC Norwood, A Assié, WH Wu, MC Renfroe, HW Song, AM Major, BS Samuel, JM Hyser, RS Longman, GE Diehl Acute high fat diet impairs macrophage supported intestinal damage resolution JCI Insight, 2023-02-08;0(0):. 2023-02-08 [PMID: 36538527]
Kanatsu-Shinohara M, Lee J, Miyazaki T et al. Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice Cell Reports Medicine [PMID: 35584625]
Hao L, Mao Y, Park J Et al. Repurposing anthelmintic praziquantel to treat psoriasis British journal of pharmacology 2021-08-07 [PMID: 34363611] (IHC-P, Mouse)	IHC-P	Mouse
Lemons K, Fu Z, Ogura T, Lin W TRPM5-expressing Microvillous Cells Regulate Region-specific Cell Proliferation and Apoptosis During Chemical Exposure Neuroscience 2020-03-26 [PMID: 32224228] (IF/IHC, Mouse)	IF/IHC	Mouse
Kanatsu-Shinohara M, Chen G, Morimoto H, Shinohara T CD2 is a surface marker for mouse and rat spermatogonial stem cells J. Reprod. Dev. 2020-03-26 [PMID: 32213736] (IF/IHC, Mouse)	IF/IHC	Mouse
Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice Nat Commun 2019-12-12 [PMID: 31831743] (IF/IHC, Mouse)	IF/IHC	Mouse
Wangler S, Peroglio M, Menzel U et al. Mesenchymal Stem Cell Homing Into Intervertebral Discs Enhances the Tie2-positive Progenitor Cell Population, Prevents Cell Death, and Induces a Proliferative Response Spine 2019-12-01 [PMID: 31730570] (IF/IHC, Human)	IF/IHC	Human
Liberia T, Martin-Lopez E, Meller SJ, Greer CA Sequential maturation of olfactory sensory neurons in the mature olfactory epithelium eNeuro 2019-09-25 [PMID: 31554664] (ICC/IF, Mouse)	ICC/IF	Mouse
Akiyama N, Yamamoto-Fukuda T, Yoshikawa M, Kojima H, Regulation of DNA methylation levels in the process of oral mucosal regeneration in a rat oral ulcer model Histol. Histopathol. 2019-07-09 [PMID: 31286466] (Rat)		Rat
Dang, TC;Ishii, Y;Nguyen, V;Yamamoto, S;Hamashima, T;Okuno, N;Nguyen, QL;Sang, Y;Ohkawa, N;Saitoh, Y;Shehata, M;Takakura, N;Fujimori, T;Inokuchi, K;Mori, H;Andrae, J;Betsholtz, C;Sasahara, M; Powerful Homeostatic Control of Oligodendroglial Lineage by PDGFR alpha in Adult Brain Cell Rep 2019-04-23 [PMID: 31018125] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Kanter JE, Kramer F, Barnhart S et al. A Novel Strategy to Prevent Advanced Atherosclerosis and Lower Blood Glucose in a Mouse Model of Metabolic Syndrome. Diabetes. 2018-02-26 [PMID: 29483182] (Mouse)		Mouse
Shinohara T, Kazuki K, Ogonuki N et al. Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice Stem Cell Reports 2017-10-10 [PMID: 28943251] (FLOW, Mouse)	FLOW	Mouse
Yoon J, Leyva-Castillo JM, Wang G et al. IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. J. Exp. Med. 2016-08-22 [PMID: 27551155] (IF/IHC, Mouse)	IF/IHC	Mouse
Liou JC, Teng MC, Tsai YS et al. UV-blocking spectacle lens protects against UV-induced decline of visual performance. Mol Vis 2015-01-01 [PMID: 26283865]
Gkouskou KK, Ioannou M, Pavlopoulos GA et al. Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis. Oncogene. 2015-08-17 [PMID: 26279300] (IF/IHC, Mouse)	IF/IHC	Mouse
He S, Zhao Z, Yang Y et al. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015-08-03 [PMID: 26234763] (IHC-P, Mouse)	IHC-P	Mouse
Rodriguez M, Siwko S, Zeng L et al. Prostate-specific G-protein-coupled receptor collaborates with loss of PTEN to promote prostate cancer progression. Oncogene 2015-06-01 [PMID: 26028029]
Talaber G, Tuckermann JP, Okret S. ACTH controls thymocyte homeostasis independent of glucocorticoids FASEB J. 2015-03-02 [PMID: 25733567] (FLOW, Mouse)	FLOW	Mouse
Kato S, Hayashi S, Kitahara Y et al. Saireito (TJ-114), a Japanese Traditional Herbal Medicine, Reduces 5-Fluorouracil-Induced Intestinal Mucositis in Mice by Inhibiting Cytokine-Mediated Apoptosis in Intestinal Crypt Cells PLoS ONE. 2015-01-08 [PMID: 25565296] (IHC-P, Human) Details: Ki-67/MKI67 antibody used for IHC-P on murine jejunum tissue sections in experiments involving the modulatory effects of a Japanese Traditional Herbal Medicine called Saireito on 5-Fluorouracil-Induced Intestinal Mucositis in Mice. IHC-P Assay involved - 10% neutralized formalin fixation of tissues, paraffin embedding followed by 4um sectioning, antigen retrieval with HistoVT One solution/90C for 20 minutes in water bath, endogenous peroxidases blocked with 3%H2O2, primary detection with Vectastain Elite ABC rabbit IgG kit followed by counter-staining with hematoxylin (Figure 6).	IHC-P	Human
Rodriguez M, Luo W, Weng J et al. PSGR promotes prostatic intraepithelial neoplasia and prostate cancer xenograft growth through NF-kB. Oncogenesis. 2014-08-12 [PMID: 25111863] (IHC-P, Mouse)	IHC-P	Mouse
Ifkovits JL, Addis RC, Epstein JA. Inhibition of TGFB Signaling Increases Direct Conversion of Fibroblasts to Induced Cardiomyocytes. PLoS ONE 2014-03-03 [PMID: 24586958] (ICC/IF, Mouse)	ICC/IF	Mouse
Lin DP, Chang HH, Yang LC et al. Assessment of ultraviolet B-blocking effects of weekly disposable contact lenses on corneal surface in a Mouse model. Mol Vis 2013-05-29 [PMID: 23734085] (IHC-P, Mouse)	IHC-P	Mouse
Jo M, Yun HM, Park KR et al. Lung tumor growth promoting function of peroxiredoxin 6. Free Radic Biol Med 2013-05-02 [PMID: 23643677] (WB, IHC-P, Mouse)	WB, IHC-P	Mouse
Mobley AS, Bryant AK, Richard MB et al. Age-dependent regional changes in the rostral migratory stream. Neurobiol Aging 2013-02-15 [PMID: 23419702] (ICC/IF, IHC-Fr, Mouse)	ICC/IF, IHC-Fr	Mouse
Show All 28 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	11/30/2016	View Summary Application Immunohistochemistry-Paraffin Sample Tested Skeletal muscle tissue Species Mouse Lot 050806 Comments Comments Heat mediated low pH antigen retrieval was performed on sections prior to use. Permeabilization with 0.1% triton. The antibody was diluted 1/200. An Alexa Fluor anti-rabbit 488 was used to detect Ki67 antibody.
	5 reviewed by: James Xiao	IHC-P	Mouse	04/07/2016	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse lung tissue that was exposed to cigarette smoke Species Mouse
Enlarge	5 reviewed by: Verified Customer	IHC-P	Mouse	02/06/2012	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse cornea Species Mouse

Array NB110-89719

• Ki67/MKI67 Antibodies
• Ki67/MKI67 Proteins
• Ki67/MKI67 ELISA