Ki67/MKI67 Antibody (1297A) - BSA Free

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, IF/IHC, WB.

Showing Publications 1 - 4 of 4.

Publications using NBP2-54791	Applications	Species
Hassanzadeh Makoui M, Mobini M, Khoshnoodi J et al. Novel Monoclonal Antibodies Specific for Human Ki67 and P53 Tumor Markers in Breast Cancer Tissue Samples Iranian journal of immunology : IJI 2023-05-31 [PMID: 37149778] (FLOW)	FLOW
Hou X, Dai H, Zheng y Circular RNA hsa_circ_0008896 accelerates atherosclerosis by promoting the proliferation, migration and invasion of vascular smooth muscle cells via hsa-miR-633/CDC20B (cell division cycle 20B) axis Bioengineered 2022-03-01 [PMID: 35212610] (WB, Human)	WB	Human
Aung TN, Acs B, Warrell J et al. A new tool for technical standardization of the Ki67 immunohistochemical assay Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2021-02-03 [PMID: 33536573] (IF/IHC, Mouse)	IF/IHC	Mouse
Ly C, Ferrier J, Gaudet J, Yockell-Lelievre J. Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro. Phytother Res. 2018-01-29 [PMID: 29377302] (IF/IHC, Human)	IF/IHC	Human

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-P	Mouse	05/12/2023	View Summary Application Immunohistochemistry-Paraffin Sample Tested Small intestine Species Mouse Lot CKPG032212A Comments Comments Requires at least heat-induced epitope retrieval. I used Target Retrieval Solution. Slides were placed in retrieval solution that was preheated to 95 degrees Celcius and left for 20m.

Array NBP2-54791

• Ki67/MKI67 Antibodies
• Ki67/MKI67 Proteins
• Ki67/MKI67 ELISA