![Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in IMR-32.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Western-Blot-H00007403-M05-img0005.jpg "Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in IMR-32.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 4E7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | KDM6A (NP_066963, 641 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QGLHKGQSSHSAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNILTVPETSRHTGETPNS |
| Specificity | This product is specific for Human UTX monoclonal antibody (M05), clone 4E7 [Gene ID: 7403]. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KDM6A |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
![Western Blot Jak1 Antibody (413104) [Unconjugated]](https://images.novusbio.com/images2/Jak1_MAB4260_Western_Blot_6164.jpg)
![Immunohistochemistry Jak1 Antibody (413104) [Unconjugated]](https://images.novusbio.com/images2/Jak1_MAB4260_Immunohistochemistry_7021.jpg)
![Intracellular Staining by Flow Cytometry Jak1 Antibody (413104) [Unconjugated]](https://images.novusbio.com/images2/Jak1_MAB4260_Flow_Cytometry_8448.jpg)
| Publication using H00007403-M05 | Applications | Species |
|---|---|---|
| Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (ICC/IF, Human) Details: Citation using the DyLight 405 version of this antibody. |
ICC/IF | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
reviewed by:
Verified Customer |
IF | Human | 10/20/2022 |
Summary
|
Secondary Antibodies |
Isotype Controls |
|
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (4E7) [H00007403-M05] - KDM6A Antibody was used (1:50) for labeling and microscopy analysis of the distribution of KDM6A proteins in MCF-7 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Immunocytochemistry-Immunofluorescence-H00007403-M05-img0008.jpg "Immunocytochemistry/Immunofluorescence: KDM6A Antibody (4E7) [H00007403-M05] - KDM6A Antibody was used (1:50) for labeling and microscopy analysis of the distribution of KDM6A proteins in MCF-7 cells. Image from verified customer review.")
![Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Expression in HepG2.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Western-Blot-H00007403-M05-img0002.jpg "Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Expression in HepG2.")
![Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in Raw 264.7.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Western-Blot-H00007403-M05-img0006.jpg "Western Blot: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of KDM6A expression in Raw 264.7.")
![Immunocytochemistry/Immunofluorescence: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of monoclonal antibody to KDM6A on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-Immunocytochemistry-Immunofluorescence-H00007403-M05-img0004.jpg "Immunocytochemistry/Immunofluorescence: KDM6A Antibody (4E7) [H00007403-M05] - Analysis of monoclonal antibody to KDM6A on HeLa cell. Antibody concentration 10 ug/ml")
![ELISA: KDM6A Antibody (4E7.) [H00007403-M05] - KDM6A Antibody (4E7) [H00007403-M05] - Detection limit for recombinant GST tagged KDM6A is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KDM6A-Antibody-4E7-ELISA-H00007403-M05-img0007.jpg "ELISA: KDM6A Antibody (4E7.) [H00007403-M05] - KDM6A Antibody (4E7) [H00007403-M05] - Detection limit for recombinant GST tagged KDM6A is 0.1 ng/ml as a capture antibody.")
![Western Blot EZH2/KMT6 Antibody [Unconjugated]](https://images.novusbio.com/images2/EZH2_AF4767_Western_Blot_12571.jpg)
![Western Blot EZH2/KMT6 Antibody [Unconjugated]](https://images.novusbio.com/images/af4767_human-mouse-ezh2-affinity-purified-polyclonal-ab-4120241240516.jpg)
![Western Blot EZH2/KMT6 Antibody [Unconjugated]](https://images.novusbio.com/images/af4767_human-mouse-ezh2-affinity-purified-polyclonal-ab-4120241240219.jpg)
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![Knockout Validated Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Knockout_Validated_22540.jpg)
![Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Immunocytochemistry__Immunofluorescence_19067.jpg)
![Western Blot Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]](https://images.novusbio.com/images2/Isocitrate_Dehydrogenase_1_MAB7049_Western_Blot_13853.jpg)
