Which specific peptide sequence are detected by this antibody? It is only stated in the data sheet that it reacts against a part of the C-terminal part of KIR4.1. Also, do you know if this antibody, or any of your other antibodies, could be utilized as an intracellular staining of cells for flow cytometry? Furthermore, I have seen in the data sheet for the synthetic peptide that it is not suitable for use with live cells, but is this an absolute? If this is the case, would it possible to buy a similar peptide which could be used with live cells, and if so at which prize/amount? Lastly, are the peptide in a frozen liquid form (or solid powder) with out trace of azide? When it comes to the cell lysate I'm wondering if this could be utilized with live cells or if the RIPA buffer which it is dissolved in makes this very toxic? If the last is the case, is it possible to provide the lysate in another buffer which are appropriate for live cells? Lastly, I wondered if the transfected HEK293T cells are also sold for culture?

Name: KCNJ10 Antibody
Brand: Novus Biologicals
SKU: NBP1-70371
Price: 399 USD

Question

Accepted Answer

I can disclose that the immunogen sequence of this antibody corresponds to a region within amino acids 330-379 of human KCNJ10. This antibody has not yet been tested in flow cytometry (you can see this as flow is not listed as a tested application in the product information), so we cannot guarantee that it would be suitable for this application. However, if you would be interested in testing this novel application, please take a look at our Innovator's Reward program. I have searched for an alternative product for you, but unfortunately none of our KCNJ10 antibodies currently have flow cytometry as a validated application.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Description	This peptide is soluble in PBS and water.
Immunogen	A synthetic peptide from amino acid region 330-379 of human KCNJ10 conjugated to blue carrier protein was used as the antigen.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	KCNJ10
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Lyophilized from whole antisera
Preservative	No Preservative
Concentration	LYOPH
Purity	Unpurified
Reconstitution Instructions	Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Publications using NBP1-70371	Applications	Species
Locher H, de Groot JC, van Iperen L et al. Development of the stria vascularis and potassium regulation in the human fetal cochlea: Insights into hereditary sensorineural hearing loss. Dev Neurobiol 2015-11-01 [PMID: 25663387] (IF/IHC)	IF/IHC
Milton M In vivo Analysis of kir4.1 Channel Expression Following Photothrombotic Stroke and Antidepressant Therapy Carleton University 2023-07-18 (WB, IHC, Mouse)	WB, IHC	Mouse
Milton M In vivo Analysis of kir4. 1 Channel Expression Following Photothrombotic Stroke and Antidepressant Therapy Thesis 2023-01-01 (Western Blot, Immunohistochemistry-Frozen, Mouse)	Western Blot, Immunohistochemistry-Frozen	Mouse

KCNJ10 Antibody

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