| Reactivity | Hu, Rt(-)Species Glossary |
| Applications | WB, Simple Western, IHC, CyTOF-ready |
| Clone | L1C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Janelia Fluor 549 |
| Immunogen | Human B-Lymphoma Cells |
| Localization | Cell Surface, Cytoplasmic and Secreted |
| Marker | B-Cell Marker |
| Specificity | This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGKC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Kappa Light Chain Antibody (NBP2-33142JF549)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGKC |
![Control Mouse IgG3 Isotype Control (133316) [Unconjugated]](https://images.novusbio.com/images2/IgG3_MAB007_Control_19774.jpg)
![Immunohistochemistry CCR2 Antibody (48607) [Unconjugated]](https://images.novusbio.com/images2/CCR2_MAB150_Immunohistochemistry_18879.jpg)
![Flow Cytometry CCR2 Antibody (48607) [Unconjugated]](https://images.novusbio.com/images2/CCR2_MAB150_Flow_Cytometry_19981.jpg)
![Neutralization CXCL9/MIG Antibody [Unconjugated]](https://images.novusbio.com/images2/CXCL9_AF-492-NA_Block_Neutralize_8729.jpg)
![Immunocytochemistry/ Immunofluorescence CXCL9/MIG Antibody [Unconjugated]](https://images.novusbio.com/images/af-492-na_mouse-cxcl9-mig-affinity-purified-polyclonal-ab-8220249504618.jpg)
![Western Blot CXCL9/MIG Antibody [Unconjugated]](https://images.novusbio.com/images/af-492-na_mouse-cxcl9-mig-affinity-purified-polyclonal-ab-8220249512813.jpg)
![Immunocytochemistry Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Immunocytochemistry__Immunofluorescence_20674.jpg)
![Immunohistochemistry Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Immunohistochemistry_20779.jpg)
![Western Blot Progesterone R/NR3C3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Progesterone_R_AF5415_Western_Blot_5715.jpg)
![SDS-PAGE TGF-beta 2 [Unconjugated]](https://images.novusbio.com/images2/TGF-beta_2_302-B2_268.jpg)
![Bioactivity TGF-beta 2 [Unconjugated]](https://images.novusbio.com/images2/TGF-beta_2_302-B2_269.jpg)
![Bioactivity TGF-beta 3 [Unconjugated]](https://images.novusbio.com/images/243-b3_recombinant-human-tgf-beta-3-protein-bioactivity-1342023172157.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image form verified customer review.](https://images.novusbio.com/images/Goat-anti-Human-Kappa-Light-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB7463-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image form verified customer review.")