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Ig Lambda C Domain/IGLC2 Antibody

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Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Immunoglobulin lambda constant region at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6525-SP
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Ig Lambda C Domain/IGLC2 Antibody Summary

Immunogen
E. coli-derived recombinant human IGLC2
Gln2-Ser106
Accession # P0CG05
Specificity
Detects recombinant human IGLC2 in direct ELISAs and Western blots. Detects endogenous Immunoglobulin lambda constant region in natural samples by Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGLC2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ig Lambda C Domain/IGLC2 Antibody

  • Ig Lambda C Domain
  • IGLC
  • IGLC2
  • immunoglobulin lambda constant 2 (Kern-Oz- marker)
  • MGC20392
  • MGC45681

Background

Immunoglobulin lambda constant region is a Ke-Oz- component of immunoglobulin (Ig) lambda light chains and is found C-terminal to the joining region of the light chain. IGLC2 is 105 amino acids (aa) in length. It contains one Ig-like domain (aa 7-100) and generates a disulfide bond with an IgH chain via Cys104. One potential variant shows a three aa substitution for aa 7-9. The closest mouse  lambda ortholog shares 61% aa identity with full-length human IGLC2. IGLC2 shares 93 - 98 % aa identity with IGLC1, IGLC3 and IGLC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGLC2
Uniprot