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Goat anti-Human Kappa Light Chain Secondary Antibody

 (9 Publications)
 (1 Review)
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Immunocytochemistry/Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized 0.5% Triton X-100 5min; wash PBS X 3. Block 10% donkey serum 1hr. 1:10,000 NB7463 O/N at 4C; wash PBS X 3 and 1:2000 donkey F(ab')2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour at room temp; wash PBS X 3. Olympus/BIOVIEW Cy3 filter 300ms exposure. Image form verified customer review.
Immunocytochemistry/ Immunofluorescence: Goat anti-Human Kappa Light Chain Secondary Antibody [NB7463] - JJN3 and 8226 Human myeloma cells stained with NB7463. Cells fixed in 4%PFA 10min, wash PBS X 3. Permeabilized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alkaline Phosphatase
NB7464
Allophycocyanin
NB7463APC
Biotin
NB7463B
DyLight 350
NB7463UV
DyLight 405
NB7463V
DyLight 488
NB7463G
DyLight 550
NB7463R
DyLight 594
NB7463DL594
DyLight 650
NB7463C
DyLight 680
NB7463FR
DyLight 755
NB7463IR
FITC
NB7465
HRP
NB7466
PE
NB7463PE
PerCP
NB7463PCP
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Goat anti-Human Kappa Light Chain Secondary Antibody Summary

Immunogen
Human Kappa Light Chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all human immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGKC
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:200- 1:2000
  • Western Blot 1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Reviewed Applications
Read 1 Review rated 5
using
NB7463 in the following applications:
Publications
Read Publications using NB7463.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kappa Light Chain Antibody (NB7463)(9)


Showing Publications 1 - 9 of 9.
Publications using NB7463 Applications Species
Luo G, Yogeshwar S, Lin L Et al. T cell reactivity to regulatory factor X4 in type 1 narcolepsy Sci Rep 2021-04-10 [PMID: 33837283]

Details:
Citation using the HRP version of this antibody.
Smith B, Kiessling A, Lledo-Garcia R et al. Generation and characterization of a high affinity anti-human FcRn antibody, rozanolixizumab, and the effects of different molecular formats on the reduction of plasma IgG concentration MAbs 2018-08-22 [PMID: 30130439]
Kiessling P, Lledo-Garcia R, Watanabe S et al. The FcRn inhibitor rozanolixizumab reduces human serum IgG concentration: A randomized phase 1 study Sci Transl Med 2017-11-03 [PMID: 29093180]
Richards FM, Tape CJ, Jodrell DI et al. Anti-tumour effects of a specific anti-ADAM17 antibody in an ovarian cancer model in vivo. PLoS One. 2012-01-01 [PMID: 22792380]

Details:
Citation using the HRP form of this antibody.
Wang G, Ujiie H, Shibaki A et al. Blockade of autoantibody-initiated tissue damage by using recombinant fab antibody fragments against pathogenic autoantigen. Am J Pathol. [PMID: 20042683]

Details:
Citation using the HRP form of this antibody.
Suzuki N, Aoki D, Tamada Y et al. HMOCC-1, a human monoclonal antibody that inhibits adhesion of ovarian cancer cells to human mesothelial cells. Gynecol Oncol . [PMID: 15491748]

Details:
Citation using the HRP form of this antibody.
Mukherjee J, Chios K, Fishwild D et al. Production and characterization of protective human antibodies against Shiga toxin 1. Infect Immun. [PMID: 12228326]

Details:
Citation using the HRP form of this antibody.
Mukherjee J, Chios K, Fishwild D et al. Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection. Infect Immun. [PMID: 11796590]

Details:
Citation using the HRP form of this antibody.
Tomokiyo K, Teshima K, Nakatomi Y et al. Induction of acquired factor IX inhibitors in cynomolgus monkey (Macaca fascicularis): a new primate model of hemophilia B. Thromb Res. [PMID: 11369429]

Details:
Citation using the HRP form of this antibody.

Review for Kappa Light Chain Antibody (NB7463) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7463:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Kappa Light Chain NB7463
Enlarge 		5
reviewed by:
Natalie Erdmann
 ICC Human 04/08/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedJJN3
SpeciesHuman
LotA6

Comments

Comments1. Cells fixed in 4%PFA 10 minutes, wash PBS X 3
2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3
3. Block 10% donkey serum 1 hour
4. 1/10,000 NB7463 O/N @4oC; wash PBS X 3
5. 1/2000 donkey F(ab’)2 Alexa Fluor Cy3 anti-goat IgG (H+L) 1 hour @room temp (Jackson # 705-166-147); wash PBS X 3
6. Olympus/BIOVIEW Cy3 filter 300ms exposure

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Recent Reviews

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Immunocytochemistry Kappa Light Chain NB7463
Enlarge
 Natalie Erdmann
04/08/2019
Application: ICC
Species: Human
read this review

Bioinformatics

Gene Symbol IGKC