![Western Blot: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Analysis of Raji cell Lysate using kappa light chain monoclonal Antibody (L1C1).](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-L1C1-Azide-and-BSA-Free-Western-Blot-NBP2-33142-img0003.jpg "Western Blot: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Analysis of Raji cell Lysate using kappa light chain monoclonal Antibody (L1C1).")
| Reactivity | Hu, Rt(-)Species Glossary |
| Applications | WB, Simple Western, IHC, CyTOF-ready |
| Clone | L1C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15191). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Human B-Lymphoma Cells |
| Localization | Cell Surface, Cytoplasmic and Secreted |
| Marker | B-Cell Marker |
| Specificity | This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGKC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. |
| Theoretical MW | 22.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Kappa Light Chain Antibody (NBP2-33142)Find related products by research area.
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| Gene Symbol | IGKC |
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (L1C1).](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-L1C1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-33142-img0004.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (L1C1).")
![Simple Western: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Electropherogram image of the corresponding Simple western lane view. Kappa Light Chain antibody was used at 10 ug/ml dilution on h serum, h tonsil lysate(s) respectively.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-L1C1-Azide-and-BSA-Free-Simple-Western-NBP2-33142-img0001.jpg "Simple Western: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Electropherogram image of the corresponding Simple western lane view. Kappa Light Chain antibody was used at 10 ug/ml dilution on h serum, h tonsil lysate(s) respectively.")
![Simple Western: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of h serum, h tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-L1C1-Azide-and-BSA-Free-Simple-Western-NBP2-33142-img0002.jpg "Simple Western: Kappa Light Chain Antibody (L1C1) - Azide and BSA Free [NBP2-33142] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of h serum, h tonsil lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")