Home » Kallikrein 11 » Kallikrein 11 Antibodies » Kallikrein 11 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

Kallikrein 11 Antibody [Unconjugated]

 (1 Publication)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Kallikrein 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1595) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Kallikrein 11 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Kallikrein 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1595) at 10 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1595
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1595-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Kallikrein 11 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Ile54-Asn282
Accession # Q9UBX7
Specificity
Detects human Kallikrein 11 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Kallikrein 1, rhKallikrein 3, rhKallikrein 5, and rhKallikrein 9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLK11
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1595 in the following applications:
Publications
Read Publication using
AF1595 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 11 Antibody [Unconjugated]

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.4
  • Hippostasin
  • hK11
  • Kallikrein 11
  • kallikrein-related peptidase 11
  • KLK11
  • MGC33060
  • PRSS20kallikrein-11
  • Serine protease 20
  • TLSPhippostasin
  • Trypsin-like protease

Background

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225. 
  3. Yousef, G.M. et al. (2000) Genomics 63:88.
  4. Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
  5. Gan, L. et al. (2000) Gene 257:119.
  6. Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
  7. Nakamura, T. et al. (2003) Urology 61:1042.
  8. Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Kallikrein 3/PSA
[HRP]
DKK300
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
(17 Publications)
Species: Hu
Applications: ELISA
Kallikrein
6/Neurosin Antibody
AF2008
Immunohistochemistry Kallikrein 6/Neurosin Antibody
(1 Review)
(8 Publications)
Species: Hu
Applications: IHC, IP, WB
Kallikrein 1
Antibody (323803) [Un ...
MAB2337
Kallikrein 1 Antibody (323803) [Unconjugated]
(1 Review)
Species: Hu
Applications: IP, WB

Puromycin-sensitive am ...
NBP2-13669
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Immunohistochemistry Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Protein S/PROS1
Antibody [Unconjugate
AF4036
Western Blot Protein S/PROS1 Antibody [Unconjugated]
Simple Western Protein S/PROS1 Antibody [Unconjugated]
(7 Publications)
Species: Hu
Applications: Simple Western, WB
PLAG1 Antibody
(3B7)
H00005324-M02
Immunohistochemistry PLAG1 Antibody (3B7)
Immunohistochemistry PLAG1 Antibody (3B7)
ELISA PLAG1 Antibody (3B7)
(20 Publications)
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
PSAT1 Antibody
NBP1-32920
Western Blot PSAT1 Antibody
Immunocytochemistry/ Immunofluorescence PSAT1 Antibody
Immunohistochemistry-Paraffin PSAT1 Antibody
(12 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Kallikrein 7
Antibody [Unconjugate
AF2624
Western Blot Kallikrein 7 Antibody [Unconjugated]
Immunohistochemistry Kallikrein 7 Antibody [Unconjugated]
(10 Publications)
Species: Hu, Mu
Applications: IHC, IP, WB
Kallikrein
4/Prostase/EMSP1
1719-SE
Kallikrein 4/Prostase/EMSP1
(6 Publications)
Species: Hu
Applications: EnzAct
Kallikrein 14
[Unconjugated]
2626-SE
Enzyme Activity Kallikrein 14 [Unconjugated]
(6 Publications)
Species: Hu
Applications: EnzAct
Kallikrein
8/Neuropsin [Unconjug
2025-SE
Kallikrein 8/Neuropsin [Unconjugated]
(6 Publications)
Species: Hu
Applications: EnzAct
Kallikrein 5
[Unconjugated]
1108-SE
Enzyme Activity Kallikrein 5 [Unconjugated]
(19 Publications)
Species: Hu
Applications: EnzAct
Kallikrein 2
Antibody
NBP1-06046
Western Blot Kallikrein 2 Antibody
Immunohistochemistry-Paraffin Kallikrein 2 Antibody
Immunohistochemistry-Paraffin Kallikrein 2 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
Kallikrein 10
Antibody (1G8)
H00005655-M01
Western Blot Kallikrein 10 Antibody (1G8)
Immunocytochemistry/ Immunofluorescence Kallikrein 10 Antibody (1G8)
ELISA Kallikrein 10 Antibody (1G8)
(2 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Kif2a Antibody
NB500-180
Knockout Validated Kif2a Antibody
Western Blot Kif2a Antibody
Immunocytochemistry/ Immunofluorescence Kif2a Antibody
(19 Publications)
Species: Hu, Ma, Mu, Po, Rt
Applications: ICC/IF, IP, KO, Simple Western, WB
Kv1.5 Antibody
NBP2-76939
Western Blot Kv1.5 Antibody
Immunocytochemistry/ Immunofluorescence Kv1.5 Antibody
Immunocytochemistry/ Immunofluorescence Kv1.5 Antibody
(1 Review)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
HOOK2 Antibody
NBP2-14986
Immunocytochemistry/ Immunofluorescence HOOK2 Antibody
Immunohistochemistry-Paraffin HOOK2 Antibody
Immunohistochemistry-Paraffin HOOK2 Antibody
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Hexokinase 2
Antibody (OTI4C5)
NBP2-02272
Flow Cytometry Hexokinase 2 Antibody (OTI4C5)
Immunocytochemistry/ Immunofluorescence Hexokinase 2 Antibody (OTI4C5)
Immunocytochemistry/ Immunofluorescence Hexokinase 2 Antibody (OTI4C5)
(5 Publications)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, WB
Kallikrein 13
Antibody
AF2625
Immunohistochemistry Kallikrein 13 Antibody
Species: Hu
Applications: IHC, IP, Neut, WB
Kallikrein 11
Antibody [Unconjugate
AF1595
Kallikrein 11 Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, IP

Publications for Kallikrein 11 Antibody (AF1595)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for Kallikrein 11 Antibody (AF1595) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AF1595:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IHC-P Other 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22969854
SpeciesOther

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Kallikrein 11 Antibody (AF1595) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Kallikrein 11 Products

Blogs on Kallikrein 11

There are no specific blogs for Kallikrein 11, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
 
 Verified Customer
01/05/2015
Application: IHC-P
Species: Other
read this review

Bioinformatics

Gene Symbol KLK11
Uniprot