![Western Blot: IVD Antibody (04) [NBP3-06536] - Lane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:40 kDa](http://images.novusbio.com/fullsize/IVD-Antibody-04-Western-Blot-NBP3-06536-img0004.jpg "Western Blot: IVD Antibody (04) [NBP3-06536] - Lane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:47 kDaObserved band size:40 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IP |
| Clone | 04 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IVD (Accession#: AAH17202.1; His30-His423). |
| Specificity | No cross-reactivity in ELISA with: Insect cell lysate |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IVD |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IVD Antibody (NBP3-06536)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IVD |
![Immunocytochemistry/Immunofluorescence: IVD Antibody (04) [NBP3-06536] - Staining of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IVD monoclonal antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/IVD-Antibody-04-Immunocytochemistry-Immunofluorescence-NBP3-06536-img0001.jpg "Immunocytochemistry/Immunofluorescence: IVD Antibody (04) [NBP3-06536] - Staining of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IVD monoclonal antibody (1:300) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: IVD Antibody (04) [NBP3-06536] - Analysis of Human IVD expression on HeLa cells. The cells were treated, stained with purified anti-Human IVD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IVD-Antibody-04-Flow-Cytometry-NBP3-06536-img0002.jpg "Flow Cytometry: IVD Antibody (04) [NBP3-06536] - Analysis of Human IVD expression on HeLa cells. The cells were treated, stained with purified anti-Human IVD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunoprecipitation: IVD Antibody (04) [NBP3-06536] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IVD mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IVD mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa](http://images.novusbio.com/fullsize/IVD-Antibody-04-Immunoprecipitation-NBP3-06536-img0003.jpg "Immunoprecipitation: IVD Antibody (04) [NBP3-06536] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-IVD mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-IVD mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa")
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