![Western Blot: Islet-1 Antibody [NBP2-14999] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0008.jpg "Western Blot: Islet-1 Antibody [NBP2-14999] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Ze, Bv, Ch, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Islet-1. The exact sequence is proprietary. |
| Predicted Species | Bovine (100%), Chicken (99%), Xenopus (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ISL1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Islet-1 Antibody (NBP2-14999)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Islet-1 Antibody [NBP2-14999] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14999-img0003.jpg "Immunocytochemistry/Immunofluorescence: Islet-1 Antibody [NBP2-14999] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Frozen: Islet-1 Antibody [NBP2-14999] - Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Immunohistochemistry-Frozen-NBP2-14999-img0004.jpg "Immunohistochemistry-Frozen: Islet-1 Antibody [NBP2-14999] - Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.")
![Western Blot: Islet-1 Antibody [NBP2-14999] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0002.jpg "Western Blot: Islet-1 Antibody [NBP2-14999] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Islet-1 Antibody [NBP2-14999] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0006.jpg "Western Blot: Islet-1 Antibody [NBP2-14999] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Islet-1 Antibody [NBP2-14999] - Rat fore brain. Islet 1 antibody dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Islet-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14999-img0001.jpg "Immunohistochemistry-Paraffin: Islet-1 Antibody [NBP2-14999] - Rat fore brain. Islet 1 antibody dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")