![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0011.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.")
| Reactivity | Hu, Ca, GpSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IP |
| Clone | YFC118.3 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Clone YFC118.3 was used by HLDA to establish CD designation. |
| Immunogen | Human neutrophils |
| Specificity | This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. CD18 is expressed by the majority of leucocytes. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | ITGB2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Canine | 09/04/2021 |
Summary
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reviewed by:
Verified Customer |
ICC | Other | 01/23/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Integrin beta 2/CD18 Antibody (NB200-610)Find related products by research area.
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CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a... Read full blog post. |
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| Verified Customer 09/04/2021 |
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| Application: | IHC-Fr | |
| Species: | Canine |
| Verified Customer 01/23/2015 |
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| Application: | ICC | |
| Species: | Other |
![Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunohistochemistry-Frozen-NB200-610-img0018.jpg "Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.")
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0017.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.")
![Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Immunocytochemistry-Immunofluorescence-NB200-610-img0007.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Analysis of canine thoracic fluid samples using anti-Integrin beta 2 antibody. The antibody showing positive cell surface staining. ICC/IF image submitted by a verified customer review.")
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0001.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood lymphocytes with Rat anti Human CD18.")
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0008.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.")
![Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.](http://images.novusbio.com/fullsize/Integrin-beta-2-CD18-Antibody-YFC118-3-Flow-Cytometry-NB200-610-img0015.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing high affinity conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 ug/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.")
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