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Integrin beta 2/CD18 Antibody (YFC118.3) [Allophycocyanin/Cy7]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
YFC118.3
Clonality
Monoclonal
Host
Rat
Conjugate
Allophycocyanin/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB200-610AF350
Alexa Fluor 405
NB200-610AF405
Alexa Fluor 488
NB200-610AF488
Alexa Fluor 532
NB200-610AF532
Alexa Fluor 594
NB200-610AF594
Alexa Fluor 647
NB200-610AF647
Alexa Fluor 700
NB200-610AF700
Alexa Fluor 750
NB200-610AF750
Allophycocyanin
NB200-610APC
Biotin
NB200-610B
CoraFluor 1
NB200-610CL1
DyLight 350
NB200-610UV
DyLight 405
NB200-610V
DyLight 488
NB200-610G
DyLight 550
NB200-610R
DyLight 594
NB200-610DL594
DyLight 650
NB200-610C
DyLight 680
NB200-610FR
DyLight 755
NB200-610IR
FITC
NB200-610F
HRP
NB200-610H
Janelia Fluor 525
NB200-610JF525
Janelia Fluor 549
NB200-610JF549
Janelia Fluor 585
NB200-610JF585
Janelia Fluor 635
NB200-610JF635
Janelia Fluor 646
NB200-610JF646
Janelia Fluor 669
NB200-610JF669
PE
NB200-610PE
PE/Atto594
NB200-610PEATT594
PE/Cy5.5
NB200-610PECY55
PE/Cy7
NB200-610PECY7
PerCP
NB200-610PCP
Unconjugated
NB200-610
mFluor Violet 450 SE
NB200-610MFV450
mFluor Violet 500 SE
NB200-610MFV500
mFluor Violet 610 SE
NB200-610MFV610
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Integrin beta 2/CD18 Antibody (YFC118.3) [Allophycocyanin/Cy7] Summary

Additional Information
Clone YFC118.3 was used by HLDA to establish CD designation.
Immunogen
Human neutrophils
Specificity
This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. CD18 is expressed by the majority of leucocytes.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
ITGB2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Integrin beta 2/CD18 Antibody (YFC118.3) [Allophycocyanin/Cy7]

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.
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Bioinformatics

Gene Symbol ITGB2