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Integrin beta 2/CD18 Antibody (10E12) - BSA Free

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Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) [NBP1-47440] - Confocal immunofluorescence analysis of HL60 cells using anti-CD18 monoclonal antioby (green). Blue: DRAQ5 fluorescent DNA ...read more
Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody (10E12) [NBP1-47440] - CD18 cells in mouse brain tumor tissue. Staining for antigen retrieval for 30 minutes. Primary antibody dilution is 1:200. Incubated ...read more
Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (10E12) [NBP1-47440] - Confocal immunofluorescence analysis of BCBL-1 cells (left) and L1210 cells (right) using anti-CD18 monoclonal antioby (red). ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA, ICC/IF, IHC
Clone
10E12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

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Integrin beta 2/CD18 Antibody (10E12) - BSA Free Summary

Immunogen
Purified recombinant fragment of Integrin beta 2/CD18 expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ITGB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Integrin beta 2/CD18 Antibody (10E12) is validated for IHC from a verified customer review.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47440 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Integrin beta 2/CD18 Antibody (10E12) - BSA Free

  • 959 beta subunit precursor)10
  • CD18 antigen
  • CD18
  • CD18beta 2
  • cell surface adhesion glycoprotein (LFA-1
  • CR3
  • Integrin beta 2
  • integrin beta-2
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
  • ITGB2
  • LAD
  • LCAMB
  • leukocyte cell adhesion molecule CD18
  • leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • LFA-1 beta
  • LFA-1
  • MAC-1 beta
  • MF17
  • MFI7
  • P150
  • p150,95 beta

Background

CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 2/CD18 Antibody (NBP1-47440) (0)

There are no publications for Integrin beta 2/CD18 Antibody (NBP1-47440).
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Review for Integrin beta 2/CD18 Antibody (NBP1-47440) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-47440:
Filter by Applications
IHC-P
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Mouse 09/26/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedCD18 cells in brain tumor
SpeciesMouse
Lot10E12

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Integrin beta 2/CD18 Antibody (NBP1-47440). (Showing 1 - 1 of 1 FAQ).

  1. Is this antibody (Anti-CD18 antibody 10E12) blocking the CD18 function?
    • Unfortunately, our Integrin beta 2/CD18 antibody has not yet been tested in a blocking/neutralizing assay. We do not have any information available on this antibody's use in that specific application and cannot guarantee its performance in it.

Secondary Antibodies

 

Isotype Controls

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Research Areas for Integrin beta 2/CD18 Antibody (NBP1-47440)

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Blogs on Integrin beta 2/CD18.

CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.

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Recent Reviews

5
5
1
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2
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1
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Verified Customer
09/26/2021
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol ITGB2