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iNOS Antibody (K13-A)

 (14 Publications)
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Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um sections) stained according to IHC-P Protocol for NBP1-33780.
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Liver tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human tissues (4 um ...read more
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Lung adenocarcinoma tissue stained with anti-iNOS antibody shows strong positive immunostaining of hepatocytes. Formalin fixed, paraffin embedded human ...read more
Immunohistochemistry-Paraffin: iNOS Antibody (K13-A) [NBP1-33780] - Inflamed porcine intestine. Antibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation. Image from verified ...read more
Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) and CD86 (B), and M2 marker: Arginase-1 (C) on the mouse paw muscle 48 h after the model induction ...read more
Western Blot: iNOS Antibody (K13-A) [NBP1-33780] - Effects of omega-3 supplementation on the immunocontent of macrophages M1 markers: nitric oxide synthase 2 (NOS-2) (A) & CD86 (B), & M2 marker: Arginase-1 (C) on the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC
Clone
K13-A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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iNOS Antibody (K13-A) Summary

Description
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from human iNOS sequence. Antibody recognizes the epitope between Ser1118 - Gly1129.
Epitope
Ser1118 - Gly1129
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NOS2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:2000
Application Notes
Use in IHC-P reported in scientific literature (PMID:34496231).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-33780 in the following applications:
Publications
Read Publications using
NBP1-33780 in the following applications:

Reactivity Notes

This antibody also recognizes nNOS (neuronal nitric oxide synthase). Porcine reactivity reported per verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for iNOS Antibody (K13-A)

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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iNOS Antibody
(K13-A)
NBP1-33780
iNOS Antibody (K13-A)
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Applications: WB, IHC

Publications for iNOS Antibody (NBP1-33780)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, KD, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(3)
KD
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-33780 Applications Species
Li X, Ke Y, Hernandez AL et al. Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGF?-dependent squamous cell carcinoma lung metastasis Cancer Letters 2023-07-01 [PMID: 37524225] (IHC-P, Mouse)

Details:
1:500 IHC-P dilution 		IHC-P Mouse
Sun A, Lin J, Wang L et al. Involvement of iNOS-induced Reactive Enteric Glia Cells in Gastrointestinal Motility Disorders of Postoperative Ileus Mice Journal of chemical neuroanatomy 2023-07-15 [PMID: 37459999] (IHC, Mouse) IHC Mouse
Salm DC, Horewicz VV, Tanaka F et al. Electrical Stimulation of the Auricular Branch Vagus Nerve Using Random and Alternating Frequencies Triggers a Rapid Onset and Pronounced Antihyperalgesia via Peripheral Annexin A1-Formyl Peptide Receptor 2/ALX Pathway in a Mouse Model of Persistent Inflammatory Pain Molecular neurobiology 2023-02-06 [PMID: 36745336]
Paula Franson Fernandes, Taynah de Oliveira Galassi, Verônica Vargas Horewicz, Afonso Shiguemi Inoue Salgado, Josiel Mileno Mack, Heloiza dos Santos Baldança, Ana Paula Ferreira da Silva, Stephen Bruehl, Edsel B. Bittencourt, Lynsey A. Seim, Daniel Fernandes Martins, Franciane Bobinski Immunoregulatory Effect of Preventive Supplementation of Omega-3 Fatty Acid in a Complex Regional Pain Syndrome Type I Model in Mice Frontiers in Integrative Neuroscience 2022-03-22 [PMID: 35391753]
Omura CM, LUdtke DD, Horewicz VV et al. Decrease of IL-1 beta and TNF in the Spinal Cord Mediates Analgesia Produced by Ankle Joint Mobilization in Complete Freund Adjuvant-Induced Inflammation Mice Model Frontiers in physiology 2022-01-14 [PMID: 35095573] (WB, Mouse) WB Mouse
Chung AW, Anand K, Anselme AC et al. A phase 1/2 clinical trial of the nitric oxide synthase inhibitor L-NMMA and taxane for treating chemoresistant triple-negative breast cancer Science translational medicine 2021-12-15 [PMID: 34910551]
Anderson-Baucum E, PiNeros AR, Kulkarni A et al. Deoxyhypusine synthase promotes a pro-inflammatory macrophage phenotype Cell metabolism 2021-09-07 [PMID: 34496231] (IHC-P, Mouse) IHC-P Mouse
de Brito RN, Ludtke DD, de Oliveira BH et al. Balneotherapy decreases mechanical hyperalgesia by reversing BDNF and NOS2 immunocontent in spinal cord of mice with neuropathic pain J. Neuroimmunol. 2020-08-16 [PMID: 32862113]
Lee M, Wang C, Jin SW et al. Expression of human inducible nitric oxide synthase in response to cytokines is regulated by hypoxia-inducible factor-1 Free Radic. Biol. Med. 2019-01-01 [PMID: 30391674] (WB, Human) WB Human
Yan J, Tie G, Wang S et al. Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages Nat Commun 2018-01-02 [PMID: 29295997] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 14 Publications.

Review for iNOS Antibody (NBP1-33780) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Porcine.

Reviews using NBP1-33780:
Filter by Applications
IHC-P
(1)
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Filter by Species
Porcine
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin iNOS NBP1-33780
Enlarge 		5
reviewed by:
Sandra Lourenssen
 IHC-P Porcine 04/18/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedparaffin sections of inflamed intestine
SpeciesPorcine
LotDB003-02-06B

Comments

CommentsAntibody dilution 1:200, overnight 4C. Secondary anti-rabbit Alexa 488 (1:1000) 2 hr incubation.

Product General Protocols

View specific protocols for iNOS Antibody (NBP1-33780): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP1-33780). (Showing 1 - 3 of 3 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. Hi, I would like to know if this antibody (NBP1-33780) works on mouse tissue in Immunohistochemistry. Did you test the mouse cross-reactivity on IHC ?
    • At this time we have only tested NBP1-33780 on human tissues in IHC, but we do expect it to work on mouse tissues in IHC based on sequence homology.
  3. What is the concentration of NBP1-33780?
    • The concentration of the lot number DB003-02-5B is 4.9 mg/ml

Secondary Antibodies

 

Isotype Controls

Additional iNOS Products

Research Areas for iNOS Antibody (NBP1-33780)

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Blogs on iNOS.

Check out the latest blog posts on iNOS.
Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.
The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.
iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Recent Reviews

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Immunohistochemistry-Paraffin iNOS NBP1-33780
Enlarge
 Sandra Lourenssen
04/18/2018
Application: IHC-P
Species: Porcine
read this review

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot