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Human IL-27 ELISA Kit (Colorimetric)

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ELISA: Human IL-27 ELISA Kit (Colorimetric) [NBP3-06784] - Samples were spiked with high concentrations of Human IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human IL-27 ELISA Kit (Colorimetric) [NBP3-06784] - Samples were spiked with high concentrations of Human IL-27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human IL-27 ELISA Kit (Colorimetric) [NBP3-06784] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL

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Human IL-27 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human IL-27 in samples. No significant cross-reactivity or interference between Human IL-27 and analogues was observed.
Standard Curve Range
31.25 - 2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.34
Intra-Assay
5.13
Spike Recovery
93 - 110 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL27

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human IL-27 ELISA Kit (Colorimetric)

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Human IL-27 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol IL27