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IL-27 Antibody (355025) [PerCP]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
355025
Clonality
Monoclonal
Host
Rat
Conjugate
PerCP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC18342U-100UG
Alexa Fluor 405
IC18342V-100UG
Alexa Fluor 488
IC18342G-100UG
Alexa Fluor 532
FAB18342X
Alexa Fluor 594
IC18342T-100UG
Alexa Fluor 647
IC18342R-100UG
Alexa Fluor 700
IC18342N-100UG
Alexa Fluor 750
IC18342S-100UG
Allophycocyanin
FAB18342A
Biotin
FAB18342B
CoraFluor 1
FAB18342CL1
DyLight 350
FAB18342D
DyLight 405
FAB18342E
DyLight 488
FAB18342K
DyLight 550
FAB18342L
DyLight 594
FAB18342M
DyLight 650
FAB18342W
DyLight 680
FAB18342Y
DyLight 755
FAB18342Z
FITC
FAB18342F
HRP
FAB18342H
Janelia Fluor 525
FAB18342JF525
Janelia Fluor 549
FAB18342I
Janelia Fluor 646
FAB18342J
Janelia Fluor 669
FAB18342JF669
PE
FAB18342P
Unconjugated
MAB18342
mFluor Violet 450 SE
FAB18342MFV450
mFluor Violet 500 SE
FAB18342MFV500
mFluor Violet 610 SE
FAB18342MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB18342
Carrier Free
MAB18342
Carrier Free
MAB18342-SP
Carrier Free
IC18342V-100UG
Carrier Free
IC18342U-100UG
Carrier Free
IC18342T-100UG
Carrier Free
IC18342S-100UG
Carrier Free
IC18342R-100UG
Carrier Free
IC18342N-100UG
Carrier Free
IC18342G-100UG
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IL-27 Antibody (355025) [PerCP] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer
Tyr19-Pro228 (EBI3), Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Specificity
Detects mouse IL-27 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) IL‑27 p28, rmIL‑27 EBI3, recombinant human (rh) IL-27 p28, and rhIL-27 EBI3 is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL27
Purity
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Alternate Names for IL-27 Antibody (355025) [PerCP]

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL27