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IL-22 Antibody

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Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed ...read more
IL-22 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using 10 µg/mL Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Recombinant Human IL‑22 (Catalog # 782-IL) stimulates IL‑10 secretion in the COLO 205 human colorectal adeno­carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‑10 DuoSet ...read more
Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.(A) IL-22 positive cells mainly located in portal areas with brownish-yellow color by immunohistochemistry. (B) ...read more
Distribution, expression and correlation of IL-22 cells with liver fibrosis in the liver.(A) IL-22 positive cells mainly located in portal areas with brownish-yellow color by immunohistochemistry. (B) ...read more
IL-22 and IL-22R1 are expressed in human brain. IL-22, IL-22R1, GFAP, and Caveolin-1 immunohistochemistry peroxidase stainings of brain tissue sections of control (a) and MS (b–d) patients. MOG and HE stainings were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF782
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF782-SP
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IL-22 Antibody Summary

Immunogen
E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6
Specificity
Detects human, recombinant mouse, and recombinant rat IL-22 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human IL-10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL22
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF782 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 Antibody

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL22
  • IL-22
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18

Background

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta , share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-22 Antibody (AF782)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
Neutralization
(1)
All Applications
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Human
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF782 Applications Species
Xiuxing Liu, Jianjie Lv, Huishi Wang, Yingfeng Zheng, Wenru Su Functional analysis of human circulating immune cells based on high-dimensional mass cytometry STAR Protocols 2022-06-01 [PMID: 35434658]
Tingting Ren, Jingyuan Xiong, Guangliang Liu, Shaoyong Wang, Zhongqi Tan, Bin Fu et al. Imbalance of Th22/Treg cells causes microinflammation in uremic patients undergoing hemodialysis Bioscience Reports 2019-10-30 [PMID: 31427482]
S Sawamura, M Jinnin, K Inoue, K Yamane, N Honda, I Kajihara, T Makino, S Masuguchi, S Fukushima, H Ihn Regulatory mechanisms of collagen expression by interleukin-22 signaling in scleroderma fibroblasts J. Dermatol. Sci., 2017-12-29;0(0):. 2017-12-29 [PMID: 29336866] (IHC-P, Human) IHC-P Human
John A Carucci Interleukin-22 and Cyclosporine in Aggressive Cutaneous Squamous Cell Carcinoma Dermatol Clin, 2017-01-01;35(1):73-84. 2017-01-01 [PMID: 27890239] (IHC, Human) IHC Human
Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes. J Neuroinflammation, 2015-06-16;12(0):119. 2015-06-16 [PMID: 26077779] (IHC, Human) IHC Human
Jun Chen, Jinqiu Feng, Xiangdong Chen, Hui Xu, Zengtong Zhou, Xuemin Shen et al. Immunoexpression of Interleukin-22 and Interleukin-23 in Oral and Cutaneous Lichen Planus Lesions: A Preliminary Study Mediators of Inflammation 2013-11-26 [PMID: 24376306]
Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129. 2012-07-11 [PMID: 22795370] (IHC, Human) IHC Human
Serelli-Lee V, Ling KL, Ho C, Yeong LH, Lim GK, Ho B, Wong SB Persistent Helicobacter pylori Specific Th17 Responses in Patients with Past H. pylori Infection Are Associated with Elevated Gastric Mucosal IL-1β. PLoS ONE, 2012-06-25;7(6):e39199. 2012-06-25 [PMID: 22761739] (IHC-Fr, Human) IHC-Fr Human
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R IL-22 increases the innate immunity of tissues. Immunity, 2004-08-01;21(2):241-54. 2004-08-01 [PMID: 15308104] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol IL22
Uniprot