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IL-21 Antibody [Unconjugated]

 (80 Publications)
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IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room ...read more
Recombinant Mouse IL-21 (Catalog # 594-ML) stimulates proliferation in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-21 (10 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF594
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF594-SP
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IL-21 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17
Specificity
Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL21
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.2-2 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse IL-21 Antibody (Catalog # AF594)
ELISA Detection: Mouse IL-21 Biotinylated Antibody (Catalog # BAF594)
Standard: Recombinant Mouse IL-21 (Catalog # 594-ML)
Publications
Read Publications using
AF594 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-21 Antibody [Unconjugated]

  • CVID11
  • IL21
  • IL-21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform
  • Za11

Background

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha . Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) PNAS 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-21 Antibody
[Unconjugated]
AF594
IL-21 Antibody [Unconjugated]
Species: Mu
Applications: WB, B/N, ELISA(Cap), ICC/IF

Publications for IL-21 Antibody (AF594)(80)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 8 applications: ELISA Capture, ELISA Development, ELISpot Development, Flow Cytometry, ICC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
ELISA Capture
(1)
ELISA Development
(3)
ELISpot Development
(1)
Flow Cytometry
(2)
ICC
(1)
IHC-Fr
(1)
Neutralization
(5)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(14)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using AF594 Applications Species
Akgul, A;Freguia, CF;Maddaloni, M;Hoffman, C;Voigt, A;Nguyen, CQ;Fanger, NA;Fanger, GR;Pascual, DW; Treatment with a Lactococcus lactis that chromosomally express E. coli cfaI mitigates salivary flow loss in a Sjögren's syndrome-like disease Scientific reports 2023-11-09 [PMID: 37945636] (ELISA Capture, Mouse) ELISA Capture Mouse
Matsumoto D, Toba H, Kenzaki K et al. Lung regeneration with rat fetal lung implantation and promotion of alveolar stem cell differentiation by corticosteroids Regen Ther 2023-09-14 [PMID: 37744680]
Matsumoto D, Toba H, Kenzaki K et al. Lung regeneration with rat fetal lung implantation and promotion of alveolar stem cell differentiation by corticosteroids Regen Ther 9/14/2023 [PMID: 37744680]
Roux I, Fenollar-Ferrer C, Lee HJ et al. CHD7 variants associated with hearing loss and enlargement of the vestibular aqueduct Hum Genet 9/5/2023 [PMID: 37668839]
Roux I, Fenollar-Ferrer C, Lee HJ et al. CHD7 variants associated with hearing loss and enlargement of the vestibular aqueduct Hum Genet 2023-09-05 [PMID: 37668839]
Freitas-Andrade, M;Comin, CH;Van Dyken, P;Ouellette, J;Raman-Nair, J;Blakeley, N;Liu, QY;Leclerc, S;Pan, Y;Liu, Z;Carrier, M;Thakur, K;Savard, A;Rurak, GM;Tremblay, MÈ;Salmaso, N;da F Costa, L;Coppola, G;Lacoste, B; Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation Nature communications 8/16/2023 [PMID: 37587100]
Freitas-Andrade, M;Comin, CH;Van Dyken, P;Ouellette, J;Raman-Nair, J;Blakeley, N;Liu, QY;Leclerc, S;Pan, Y;Liu, Z;Carrier, M;Thakur, K;Savard, A;Rurak, GM;Tremblay, MÈ;Salmaso, N;da F Costa, L;Coppola, G;Lacoste, B; Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation Nature communications 2023-08-16 [PMID: 37587100]
Diana Cadena Castaneda, Sonia Jangra, Marina Yurieva, Jan Martinek, Megan Callender, Matthew Coxe et al. Spatiotemporally organized immunomodulatory response to SARS-CoV-2 virus in primary human broncho-alveolar epithelia iScience 8/18/2023 [PMID: 37520727]
Oyler BL, Valencia-Dávila JA, Moysi E et al. Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches iScience 8/18/2023 [PMID: 37520703]
Ugur M, Labios RJ, Fenton C et al. Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks Immunity 7/7/2023 [PMID: 37463581]

Details:
Alexa Fluor 700 conjugation used
Show All 80 Publications.

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Bioinformatics

Gene Symbol IL21
Uniprot