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IL-22 Antibody (142928) [Allophycocyanin]

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 (10 Reviews)
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CD4+Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342), 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL), 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL), and 10 ng/mL Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) for 5 to 7 days, then re-stimulated with 50 ng/mL PMA and 200 ng/mL Calcium ionomycin for 2 to 4 hours or (B) resting CD4+PBMCs were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human IL-22 APC-conjugated Monoclonal Antibody (Catalog # IC7821A). Quadrant markers were set based on isotype control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
CD4+Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal ...read more
Frequency of IL-17 and/or IL-22 expressing mucosal CD4+T cells decreases by progression of Fiebig stage.To quantify the expression of Il-17 and IL-22 in CD4+T cells, mucosal and peripheral mononuclear cells were ...read more
Frequency of IL-17 and/or IL-22 expressing mucosal CD4+T cells decreases by progression of Fiebig stage.To quantify the expression of Il-17 and IL-22 in CD4+T cells, mucosal and peripheral mononuclear cells were ...read more
Frequency of IL-17 and/or IL-22 expressing mucosal CD4+T cells decreases by progression of Fiebig stage.To quantify the expression of Il-17 and IL-22 in CD4+T cells, mucosal and peripheral mononuclear cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
142928
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
IC7821A-025
Biotin
BAM7821
Phycoerythrin
IC7821P-100
Phycoerythrin
IC7821P-025
Unconjugated
MAB7821-100
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB7821-100
Carrier Free
IC7821P-100
Carrier Free
IC7821A-025
Carrier Free
IC7821P-025
Carrier Free
MAB7821-SP
Carrier Free
MAB7821-500
Carrier Free
MAB7821-100
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IL-22 Antibody (142928) [Allophycocyanin] Summary

Immunogen
E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6
Specificity
Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL22
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 10 Reviews rated 3.8
using
IC7821A in the following application:
Publications
Read Publications using
IC7821A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 Antibody (142928) [Allophycocyanin]

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL22
  • IL-22
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18

Background

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta , share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-22 Antibody (IC7821A)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, IHC-Fr.


Filter By Application
Flow Cytometry
(11)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(11)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using IC7821A Applications Species
A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma Biomedicines, 2021-12-16;9(12):. 2021-12-16 [PMID: 34944746] (Flow Cytometry, Human) Flow Cytometry Human
Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y, Wu C Mycobacterium tuberculosis-Specific IL-21+IFN-gamma+CD4+ T Cells Are Regulated by IL-12. PLoS ONE, 2016-01-19;11(1):e0147356. 2016-01-19 [PMID: 26785168] (Flow Cytometry, Human) Flow Cytometry Human
Hong M, Sandalova E, Low D, Gehring A, Fieni S, Amadei B, Urbani S, Chong Y, Guccione E, Bertoletti A Trained immunity in newborn infants of HBV-infected mothers. Nat Commun, 2015-03-25;6(0):6588. 2015-03-25 [PMID: 25807344] (Flow Cytometry, IHC-Fr, Human) Flow Cytometry, IHC-Fr Human
Schuetz A, Deleage C, Sereti I, Rerknimitr R, Phanuphak N, Phuang-Ngern Y, Estes J, Sandler N, Sukhumvittaya S, Marovich M, Jongrakthaitae S, Akapirat S, Fletscher J, Kroon E, Dewar R, Trichavaroj R, Chomchey N, Douek D, O Connell R, Ngauy V, Robb M, Phanuphak P, Michael N, Excler J, Kim J, de Souza M, Ananworanich J Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation. PLoS Pathog, 2014-12-11;10(12):e1004543. 2014-12-11 [PMID: 25503054] (Flow Cytometry, Human) Flow Cytometry Human
Staumont-Salle D, Fleury S, Lazzari A, Molendi-Coste O, Hornez N, Lavogiez C, Kanda A, Wartelle J, Fries A, Pennino D, Mionnet C, Prawitt J, Bouchaert E, Delaporte E, Glaichenhaus N, Staels B, Julia V, Dombrowicz D CX(3)CL1 (fractalkine) and its receptor CX(3)CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin. J Exp Med, 2014-05-12;211(6):1185-96. 2014-05-12 [PMID: 24821910] (Flow Cytometry, Human) Flow Cytometry Human
Tang , Xin-Zi, Jo , Juandy, Tan , Anthony, Sandalova , Elena, Chia , Adeline, Tan , Kai Chah, Lee , Kang Hoe, Gehring , Adam J, De Libero , Gennaro, Bertoletti , Antonio IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells. J Immunol, 2013-02-27;190(7):3142-52. 2013-02-27 [PMID: 23447689] (Flow Cytometry, Human) Flow Cytometry Human
Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope. PLoS Pathog, 2012-11-29;8(11):e1003042. 2012-11-29 [PMID: 23209413] (Flow Cytometry, Human) Flow Cytometry Human
Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients. J Allergy Clin Immunol, 2012-11-19;131(2):562-70. 2012-11-19 [PMID: 23174657] (Flow Cytometry, Human) Flow Cytometry Human
Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129. 2012-07-11 [PMID: 22795370] (Flow Cytometry, Human) Flow Cytometry Human
Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection. Scand. J. Immunol., 2011-04-01;73(4):330-7. 2011-04-01 [PMID: 21223348] (Flow Cytometry, Human) Flow Cytometry Human
Show All 11 Publications.

Reviews for IL-22 Antibody (IC7821A) (10) 3.810

Average Rating: 3.8
(Based on 10 reviews)
We have 10 reviews tested in 2 species: Human, Mouse.

Reviews using IC7821A:
Filter by Applications
Flow
(10)
All Applications
Filter by Species
Human
(8)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 Flow Mouse 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20357258
SpeciesMouse
  4
reviewed by:
Verified Customer
 Flow Mouse 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20357258
SpeciesMouse
  4
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 24324435
SpeciesHuman
  4
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 24324435
SpeciesHuman
  4
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 21206487
SpeciesHuman
  4
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 21206487
SpeciesHuman
  4
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22795370
SpeciesHuman
  4
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22795370
SpeciesHuman
  3
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 23174657
SpeciesHuman
  3
reviewed by:
Verified Customer
 Flow Human 01/28/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 23174657
SpeciesHuman

FAQs for IL-22 Antibody (IC7821A) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human IL-22 PE-conjugated Monoclonal Antibody (Catalog # IC7821P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Recent Reviews

3.8
5
0
4
8
3
2
2
0
1
0
 
 Verified Customer
01/28/2015
Application: Flow
Species: Mouse
read this review
 
 Verified Customer
01/28/2015
Application: Flow
Species: Mouse
read this review
 
 Verified Customer
01/28/2015
Application: Flow
Species: Human
read this review

Bioinformatics

Gene Symbol IL22
Uniprot