Home » IL-22 » IL-22 Antibodies » IL-22 Antibody (140301) [Phycoerythrin]

IL-22 Antibody (140301) [Phycoerythrin]

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Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554A) and (A) Rat Anti-Mouse IL-22 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC582F) and (A) Rat Anti-Mouse IL-22 PE-conjugated Monoclonal Antibody (IC582P) or (B) isotype control antibody (IC006P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.
Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) ...read more
EL4 cells were stained with Rat Anti-Mouse IL‑22 PE‑conjugated Monoclonal Antibody (Catalog # IC582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
140301
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC582U-100UG
Alexa Fluor 405
IC582V-100UG
Alexa Fluor 488
IC582G-100UG
Alexa Fluor 532
FAB582X
Alexa Fluor 594
IC582T-100UG
Alexa Fluor 647
IC582R-100UG
Alexa Fluor 700
IC582N-100UG
Alexa Fluor 750
IC582S-100UG
Allophycocyanin
FAB582A
Biotin
FAB582B
DyLight 350
FAB582D
DyLight 405
FAB582E
DyLight 488
FAB582K
DyLight 550
FAB582L
DyLight 594
FAB582M
DyLight 650
FAB582W
DyLight 680
FAB582Y
DyLight 755
FAB582Z
FITC
FAB582F
HRP
FAB582H
Janelia Fluor 525
FAB582JF525
Janelia Fluor 549
FAB582I
Janelia Fluor 646
FAB582J
Janelia Fluor 669
FAB582JF669
PerCP
FAB582C
Phycoerythrin
IC582P-025UG
Unconjugated
MAB582
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB582
Carrier Free
MAB582
Carrier Free
MAB582-SP
Carrier Free
IC582R-100UG
Carrier Free
IC582N-100UG
Carrier Free
IC582V-100UG
Carrier Free
IC582G-100UG
Carrier Free
IC582U-100UG
Carrier Free
IC582T-100UG
Carrier Free
IC582S-100UG
Carrier Free
IC582P-025UG
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IL-22 Antibody (140301) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant mouse IL-22
Leu34-Val179
Accession # Q9JJY9
Specificity
Detects mouse IL-22 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat IL-22 is observed, 15% cross-reactivity with recombinant human IL-22 is observed, and no cross-reactivity with recombinant mouse IL-10 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL22
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publications using
IC582P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 Antibody (140301) [Phycoerythrin]

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL22
  • IL-22
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18

Background

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 146 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta , share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-22 Antibody (IC582P)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(5)
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Mouse
(6)
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Showing Publications 1 - 6 of 6.
Publications using IC582P Applications Species
During acute experimental infection with the reticulotropic Trypanosoma cruzi strain Tulahuen IL-22 is induced IL-23-dependently but is dispensable for protection Sci Rep, 2016-09-21;6(0):32927. 2016-09-21 [PMID: 27650379] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Walker C, Hautefort I, Dalton J, Overweg K, Egan C, Bongaerts R, Newton D, Cruickshank S, Andrew E, Carding S Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge. PLoS ONE, 2013-12-17;8(12):e84553. 2013-12-17 [PMID: 24358364] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes. J. Immunol., 2012-09-19;189(9):4237-46. 2012-09-19 [PMID: 22993203] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Kumar P, Thakar M, Ouyang W, Malarkannan S IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection. Mucosal Immunol, 2012-06-27;6(1):69-82. 2012-06-27 [PMID: 22739232] (Mouse) Mouse
Nakajima K, Kanda T, Takaishi M, Shiga T, Miyoshi K, Nakajima H, Kamijima R, Tarutani M, Benson JM, Elloso MM, Gutshall LL, Naso MF, Iwakura Y, DiGiovanni J, Sano S Distinct Roles of IL-23 and IL-17 in the Development of Psoriasis-Like Lesions in a Mouse Model. J. Immunol., 2011-02-23;186(7):4481-9. 2011-02-23 [PMID: 21346238] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Wahl C, Wegenka UM, Leithauser F, Schirmbeck R, Reimann J IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency. J. Immunol., 2009-04-15;182(8):4521-8. 2009-04-15 [PMID: 19342625] (Flow Cytometry, Mouse) Flow Cytometry Mouse

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Bioinformatics

Gene Symbol IL22
Uniprot