Reactivity | HuSpecies Glossary |
Applications | Flow |
Clone | 729632 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Alexa Fluor 488 |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4 Ala23-Ala240 Accession # Q14773 |
Specificity | Detects human ICAM-4 in direct ELISAs. |
Source | N/A |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Mouse |
Gene | ICAM4 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Storage | Protect from light. Do not freeze.
|
Buffer | Supplied in a saline solution containing BSA and Sodium Azide. |
Preservative | Sodium Azide |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
ICAM-4 (Intercellular Adhesion Molecule 4), also known as Landsteiner-Wiener Glycoprotein and CD242, is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha 4 beta 1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.
Secondary Antibodies |
Isotype Controls |
Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.