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ICAM-4 Antibody [Unconjugated]

 
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Western blot shows lysates of human erythrocytes and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM-4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human erythrocytes and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179) followed by ...read more
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for ICAM‑4 at approximately 48 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ICAM‑4 ...read more
Human red blood cells (erythrocytes) were stained with Sheep Anti-Human ICAM‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7179, filled histogram) or isotype control antibody (Catalog # 5-001-A, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF7179-SP
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ICAM-4 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4
Ala31-Ala240
Accession # Q14773
Specificity
Detects human ICAM-4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ICAM4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-4 Antibody [Unconjugated]

  • CD242 antigen
  • CD242
  • ICAM4
  • ICAM-4
  • intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
  • intercellular adhesion molecule 4 (LW blood group)
  • intercellular adhesion molecule 4
  • intercellular adhesion molecule 4, Landsteiner-Wiener blood group
  • Landsteiner-Wiener blood group antigen a
  • Landsteiner-Wiener blood group glycoprotein
  • Landsteiner-Wiener blood group
  • LWLW blood group protein

Background

ICAM-4 (intercellular adhesion molecule 4; also Landsteiner-Wiener glycoprotein and CD242) is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha 4 beta 1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ICAM4
Uniprot