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Hydroxyacid Oxidase-1/HAO-1 Antibody

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Western Blot: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP3-16999] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Western Blot: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP3-16999] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP3-16999] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP3-16999] - Analysis in human liver and pancreas tissues using Anti-HAO1 antibody. Corresponding HAO1 RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP3-16999] - Staining of human liver shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Hydroxyacid Oxidase-1/HAO-1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAO1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Hydroxyacid Oxidase-1/HAO-1 Antibody

  • (S)-2-hydroxy-acid oxidase
  • EC 1.1.3.15
  • Glycolate oxidase
  • GOX
  • GOX1MGC142227
  • GOXMGC142225
  • HAO1
  • HAO-1
  • HAOX1
  • hydroxyacid oxidase (glycolate oxidase) 1
  • hydroxyacid oxidase 1
  • Hydroxyacid Oxidase1
  • Hydroxyacid Oxidase-1

Background

HAO1 is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HAO1