Hydroxyacid Oxidase-1/HAO-1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Analysis in human liver and endometrium tissues using NBP2-14080 antibody. Corresponding HAO1 RNA-seq data ...read more
Western Blot: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Hydroxyacid Oxidase-1/HAO-1 antibody was used on canine paraffin-embedded liver tissue at a concentration of 20ug/mL and left at 4C ...read more
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Staining of human endometrium shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Staining of human liver shows strong granular cytoplasm positivity in hepatocytes.
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Staining of human testis shows moderate granular cytoplasm positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Hydroxyacid Oxidase-1/HAO-1 Antibody [NBP2-14080] - Staining of human prostate shows no positivity in glandular cells.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Hydroxyacid Oxidase-1/HAO-1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: QHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Hydroxyacid Oxidase-1/HAO-1 Protein (NBP2-14080PEP)
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-14080 in the following applications:

Publications
Read Publication using NBP2-14080.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%). Reactivity reported in scientific literature (PMID: 24648543). Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Hydroxyacid Oxidase-1/HAO-1 Antibody

  • (S)-2-hydroxy-acid oxidase
  • EC 1.1.3.15
  • Glycolate oxidase
  • GOX
  • GOX1MGC142227
  • GOXMGC142225
  • HAO1
  • HAO-1
  • HAOX1
  • hydroxyacid oxidase (glycolate oxidase) 1
  • hydroxyacid oxidase 1
  • Hydroxyacid Oxidase1
  • Hydroxyacid Oxidase-1

Background

HAO1 is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hydroxyacid Oxidase-1/HAO-1 Antibody (NBP2-14080)(1)

Reviews for Hydroxyacid Oxidase-1/HAO-1 Antibody (NBP2-14080) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Mouse, Canine, Turtle.

Reviews using NBP2-14080:
Filter by Applications
IHC-P
(3)
All Applications
Filter by Species
Mouse
(1)
Canine
(1)
Turtle
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Vojtech Gabriel
IHC-P Turtle 05/11/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedIHC Sample Tested
SpeciesTurtle
Lot000017281
  5
reviewed by:
Hannah Wickham
IHC-P Canine 03/11/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver tissue
SpeciesCanine
Lot000017281

Comments

CommentsHAO1 was used on canine paraffin-embedded liver tissue at a concentration of 20ug/mL and left at 4C overnight. HIER was performed in Tris/EDTA buffer for two hours at 75C. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
Immunohistochemistry-Paraffin Hydroxyacid Oxidase-1/HAO-1 NBP2-14080
Enlarge
3
reviewed by:
Faye Doherty
IHC-P Mouse 07/01/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver tissue
SpeciesMouse
LotR59499

Comments

CommentsAverage Antibody when tested in Mouse Liver, has clear staining patterns, however has minor background noise within the tissue.

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FAQs for Hydroxyacid Oxidase-1/HAO-1 Antibody (NBP2-14080) (0)

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Secondary Antibodies

 

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Recent Reviews

3.7
5
1
4
0
3
2
2
0
1
0

Vojtech Gabriel
05/11/2022
Application: IHC-P
Species: Turtle

Hannah Wickham
03/11/2022
Application: IHC-P
Species: Canine

Faye Doherty
07/01/2019
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol HAO1