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HuC Antibody

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Western Blot: HuC Antibody [NBP3-13367] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HuC antibody (NBP3-13367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: HuC Antibody [NBP3-13367] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HuC antibody (NBP3-13367) diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde ...read more
Immunohistochemistry-Frozen: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse hippocampus. Green: HuC stained by HuC antibody (NBP3-13367) ...read more
Immunohistochemistry-Paraffin: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HuC stained by HuC antibody ...read more
Immunohistochemistry-Paraffin: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HuC stained by HuC antibody ...read more
Immunohistochemistry-Paraffin: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HuC stained by HuC antibody ...read more
Immunohistochemistry-Frozen: HuC Antibody [NBP3-13367] - HuC antibody detects HuC protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse hippocampus. Green: HuC stained by HuC antibody (NBP3-13367) ...read more

Product Details

Summary
Reactivity Mu, Rt, HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HuC Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse HuC. The exact sequence is proprietary.
Predicted Species
Human (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ELAVL3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for HuC Antibody

  • DKFZp547J036
  • ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
  • ELAV-like protein 3
  • Hu antigen C
  • Hu-antigen C
  • HuC
  • HUCL
  • HUCParaneoplastic cerebellar degeneration-associated antigen
  • MGC20653
  • PLE21Paraneoplastic limbic encephalitis antigen 21

Background

HuC is a member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ELAVL3