![Western Blot: HR6A/UBE2A Antibody [NB100-553] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody NB100-553 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HR6A-UBE2A-Antibody-Western-Blot-NB100-553-img0019.jpg "Western Blot: HR6A/UBE2A Antibody [NB100-553] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody NB100-553 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Mu, Rt, Bv, RbSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide which maps to a region between the N-terminus of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319). |
| Predicted Species | Rat (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UBE2A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: HR6A/UBE2A Antibody [NB100-553] - Section of human stomach carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Rad6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HR6A-UBE2A-Antibody-Immunohistochemistry-NB100-553-img0016.jpg "Immunohistochemistry-Paraffin: HR6A/UBE2A Antibody [NB100-553] - Section of human stomach carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Rad6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")