Novus Biologicals products are now on bio-techne.com

HMBS Recombinant Protein Antigen

Images

 
There are currently no images for HMBS Recombinant Protein Antigen (NBP2-33600PEP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

HMBS Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human HMBS.

Source: E. coli

Amino Acid Sequence: EFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLV

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
HMBS
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-33600.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for HMBS Recombinant Protein Antigen

  • hydroxymethylbilane synthasePre-uroporphyrinogen synthase
  • PBGDPBG-D
  • PORC
  • porphobilinogen deaminase
  • porphyria, acute; Chester type
  • UPSEC 2.5.1.61
  • uroporphyrinogen I synthase
  • uroporphyrinogen I synthetase

Background

HMBS encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89158
Species: Hu, Rt
Applications: IHC, IHC-P, Simple Western, WB
NBP2-33413
Species: Hu
Applications: IHC, IHC-P, WB
NB100-127
Species: Hu, Mu, Pm, Rt
Applications: ChIP, CHIP-SEQ, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-90202
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-26190
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
H00005498-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, KD, WB
NBP1-82580
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NBP2-59438
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP1-47492
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-92565
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NB500-317
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
NBP1-33527
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
H00011026-M01
Species: Hu
Applications: ELISA, WB
H00006908-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP3-13106
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-33600PEP
Species: Hu
Applications: AC

Publications for HMBS Recombinant Protein Antigen (NBP2-33600PEP) (0)

There are no publications for HMBS Recombinant Protein Antigen (NBP2-33600PEP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HMBS Recombinant Protein Antigen (NBP2-33600PEP) (0)

There are no reviews for HMBS Recombinant Protein Antigen (NBP2-33600PEP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for HMBS Recombinant Protein Antigen (NBP2-33600PEP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HMBS Products

Research Areas for HMBS Recombinant Protein Antigen (NBP2-33600PEP)

Find related products by research area.

Blogs on HMBS

There are no specific blogs for HMBS, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)

As an example, if the molecular weight of a compound is 197.13 g/mol and the desired concentration is 10 mM for 10 ml of water based stock solution, the required mass would be = 19.713 (value determined by this calculator).

=
x
x
g/mol

Review this Product

Be the first to review our HMBS Recombinant Protein Antigen and receive a gift card or discount.

Bioinformatics

Gene Symbol HMBS