![Western Blot: HMBS Antibody (3E8) [H00003145-M01] - Analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMBS-Antibody-3E8-Western-Blot-H00003145-M01-img0004.jpg "Western Blot: HMBS Antibody (3E8) [H00003145-M01] - Analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.Lane 1: HMBS transfected lysate(39.71 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 3E8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | HMBS (AAH00520.1, 1 a.a. ~ 361 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKIPDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH |
| Specificity | HMBS (3E8) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HMBS |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: HMBS Antibody (3E8) [H00003145-M01] - analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.](http://images.novusbio.com/fullsize/HMBS-Antibody-3E8-Western-Blot-H00003145-M01-img0001.jpg "Western Blot: HMBS Antibody (3E8) [H00003145-M01] - analysis of HMBS expression in transfected 293T cell line by HMBS monoclonal antibody (M01), clone 3E8.")
![Sandwich ELISA: HMBS Antibody (3E8) [H00003145-M01] - Detection limit for recombinant GST tagged HMBS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMBS-Antibody-3E8-Sandwich-ELISA-H00003145-M01-img0003.jpg "Sandwich ELISA: HMBS Antibody (3E8) [H00003145-M01] - Detection limit for recombinant GST tagged HMBS is approximately 1ng/ml as a capture antibody.")
