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Histone H1 Antibody

Images

 
Western Blot: Histone H1 Antibody [NB100-748] - The blot shows the antibody reactiving against porcine Histone H1. (Lane 1: negative control). Zhu G et al. j. Biol. Chem. 2005, 280:23057-23065

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

Order Details

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Histone H1 Antibody Summary

Immunogen
Calf thymus intact Histone H1 (MW 23 kDa) complexed to rRNA.
Localization
Nuclear
Marker
Nuclear Marker
Specificity
This specifically binds to the Histone H1 protein found in nucleosomes of all eukaryotic cells. Differences between H1 subfractions of one species are greater than the differences across species. No cross reactivity was observed with other Histone core proteins H2A, H2B, H3 or H4.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
H1-1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:20
  • Western Blot 1:300
Application Notes
Optimal dilutions/concentrations should be determined by the end user.
Publications
Read Publication using
NB100-748 in the following applications:

  • WB
    1 publication

Reactivity Notes

Cross-reacts with all Mammals.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Histone H1 Antibody

  • H1 histone family, member 1
  • H1.1
  • H1A
  • H1F1HIST1
  • histone 1, H1a
  • histone cluster 1, H1a
  • histone H1.1
  • MGC126642
  • MGC138345

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H1 Antibody (NB100-748)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Histone H1 Antibody (NB100-748) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Histone H1 Antibody (NB100-748)

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Blogs on Histone H1.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

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Bioinformatics

Gene Symbol H1-1