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Histone H1.5 Antibody

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Immunohistochemistry-Paraffin: Histone H1.5 Antibody [NBP3-17162] - Staining of human tonsil shows strong nuclear positivity in germinal- and non germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Histone H1.5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ETATPAPVEKSPAKKKATKKAAG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H1B
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Histone H1.5 Antibody

  • H1 histone family, member 5
  • H1
  • H1.5
  • H1F5H1B
  • histone 1, H1b
  • histone cluster 1, H1b
  • histone H1.5
  • Histone H1a
  • MGC126630
  • MGC126632

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H1.5 is a 207 amino acid long, 22 kDA protein encoded by the gene HIST1H1B (found on chromosome 6p22-p21.3) that lacks polyA tails and instead obtain palindromic termination elements. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1B has been investigated regarding breast cancer, measles, ataxia, t-cell leukemia, arthritis, alzheimer's diseases, lupus, and prostate cancer. It is active in apoptosis, histone modification, PKA signaling, and cell cycle chromosome condensation in prometaphase as it interacts with genes H3F3A, H3F3B, SIRT1, CDK2, and L3MNTL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIST1H1B