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SMCP Antibody (5C10D8) - BSA Free

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Western Blot: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis using SMCP mAb against HEK293 (1) and SMCP (AA: FULL(1-116))-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis of Hela cells using SMCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western Blot: SMCP Antibody (5C10D8) [NBP2-37253] - Analysis using SMCP mAb against human SMCP (AA: FULL(1-116)) recombinant protein. (Expected MW is 38.3 kDa)
ELISA: SMCP Antibody (5C10D8) [NBP2-37253] - Black line: Control Antigen (100 ng); Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5C10D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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SMCP Antibody (5C10D8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human SMCP (AA: FULL(1-116)) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMCP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
12.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SMCP Antibody (5C10D8) - BSA Free

  • HSMCSGEN1
  • MCSMGC26305
  • MCSPMGC26519
  • mitochondrial capsule selenoprotein
  • sperm mitochondria-associated cysteine-rich protein
  • sperm mitochondrial-associated cysteine-rich protein

Background

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated,flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. Theprotein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thoughtto function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SMCP