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hHpr1-p84-Thoc1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining in human testis and skeletal muscle tissues using anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody NBP1-89670 (A) shows ...read more
Independent Antibodies: Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Analysis using Anti-THOC1 antibody NBP1-89670 (A) shows similar pattern to independent antibody NBP1-89669 (B).
Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cell line U-251 MG shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human testis using Anti-THOC1 antibody NBP1-89670.
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human colon.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human skeletal muscle using Anti-THOC1 antibody NBP1-89670.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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hHpr1-p84-Thoc1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMVENEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
THOC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
hHpr1-p84-Thoc1 Protein (NBP1-89670PEP)
Publications
Read Publication using NBP1-89670.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for hHpr1-p84-Thoc1 Antibody

  • HPR1THO complex subunit 1
  • hTREX84
  • Nuclear matrix protein p84
  • P84
  • P84N5
  • THO complex 1
  • tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hHpr1-p84-Thoc1 Antibody (NBP1-89670)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol THOC1