Home » SIRP alpha/CD172a » SIRP alpha/CD172a Antibodies » SIRP alpha/CD172a Antibody (OX-41)

SIRP alpha/CD172a Antibody (OX-41)

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Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power
Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat ...read more
Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat ...read more
Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat ...read more
Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat ...read more
Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with ...read more
Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with ...read more
Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OX-41
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65530AF350
Alexa Fluor 405
NB100-65530AF405
Alexa Fluor 488
NB100-65530AF488
Alexa Fluor 532
NB100-65530AF532
Alexa Fluor 594
NB100-65530AF594
Alexa Fluor 647
NB100-65530AF647
Alexa Fluor 700
NB100-65530AF700
Alexa Fluor 750
NB100-65530AF750
Allophycocyanin
NB100-65530APC
Biotin
NB100-65530B
DyLight 350
NB100-65530UV
DyLight 405
NB100-65530V
DyLight 488
NB100-65530G
DyLight 550
NB100-65530R
DyLight 594
NB100-65530DL594
DyLight 650
NB100-65530C
DyLight 680
NB100-65530FR
DyLight 755
NB100-65530IR
FITC
NB100-63488
FITC
NB100-65530F
HRP
NB100-65530H
Janelia Fluor 525
NB100-65530JF525
Janelia Fluor 549
NB100-65530JF549
Janelia Fluor 646
NB100-65530JF646
Janelia Fluor 669
NB100-65530JF669
PE
NB100-65530PE
PerCP
NB100-65530PCP
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SIRP alpha/CD172a Antibody (OX-41) Summary

Immunogen
Rat peritoneal macrophages
Localization
Membrane; single pass type I membrane protein.
Specificity
NB100-65530 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SIRPA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:50
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
This product requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase. Use in ICC/IF reported in scientific literature (PMID:34413330).
Publications
Read Publications using
NB100-65530 in the following applications:

  • FLOW
    3 publications

Reactivity Notes

Reacts with Rat. Use in Mouse reported in scientific literature (PMID:34413330)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SIRP alpha/CD172a Antibody (OX-41)

  • BIT
  • BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
  • Brain Ig-like molecule with tyrosine-based activation motifs
  • CD172 antigen-like family member A
  • CD172a antigen
  • CD172a
  • Inhibitory receptor SHPS-1
  • Macrophage fusion receptor
  • MFR
  • MFRtyrosine phosphatase SHP substrate 1
  • MyD-1 antigen
  • MYD1
  • MYD-1
  • P84
  • protein tyrosine phosphatase, non-receptor type substrate 1
  • PTPNS1
  • SHP substrate 1
  • SHPS1
  • SHPS-1
  • SHPS1CD172A
  • signal-regulatory protein alpha
  • Signal-regulatory protein alpha-1
  • Signal-regulatory protein alpha-2
  • Signal-regulatory protein alpha-3
  • SIRP alpha
  • SIRPA
  • SIRPalpha
  • Sirp-alpha-1
  • SIRPalpha2
  • Sirp-alpha-2
  • Sirp-alpha-3
  • SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIRP alpha/CD172a Antibody (NB100-65530)(5)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: FLOW.


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Bioinformatics

Gene Symbol SIRPA
Uniprot